细胞因子诱导的杀伤细胞培养及其在血液系统恶性肿瘤中的研究进展

Research progress of cultivation of cytokine induced killer cells and their application in treating hematologic malignancies

细胞因子诱导的杀伤细胞（CIK细胞）是人类外周血单个核细胞（PBMC）在体外经干扰素-γ、抗CD3单克隆抗体、白细胞介素（IL）-2、IL-1等诱导培养而获得的一种新型免疫活性细胞.CIK细胞具有T细胞和NK细胞的双重表面标志,在体内外拥有强大的抗肿瘤活性,且兼有非主要组织相容性复合体（MHC）限制性的优点,是目前过继免疫治疗肿瘤的主要效应细胞,已被广泛应用于肝癌、肺癌等实体瘤及白血病、淋巴瘤、骨髓瘤等血液系统肿瘤的辅助治疗.文章就CIK细胞的优化培养及在血液系统恶性肿瘤治疗中的应用进展作一综述.

Cytokine induced killer （CIK） cells are a new kind of cytotoxic cells,which are generated by stimulation of peripheral blood mononuclear cells （PBMC） with IFN-γ,IL-2,IL-1 and anti-CD3 in vitro.These cells represent phenotype of both T cell and NK cell and have advantages of the non MHC restriction,so they have a strong ability of antitumor activity in vivo and vitro.Now,CIK cells as the main effector cells of adoptive immunotherapy have been extensively used in a variety of solid tumors such as liver cancer,lung cancer and hematological malignancies including leukemia,lymphoma,myeloma.In this article,the optimized cultivation of CIK cells as well as their most prominent clinical application in hematologic malignancies were summarized.