MMP-7、MMP-26在妊娠滋养细胞疾病中的表达研究

Expressions of MMP-7 and MMP-26 in gestational trophoblastic disease

目的研究基质金属蛋白酶(MMP)-7、MMP-26在滋养细胞疾病中的表达及与研究滋养细胞肿瘤相关因素之间的关系,为早期临床诊断滋养细胞疾病,判断其恶性程度提供有价值的线索。方法应用免疫组织化学S-ABC法检测10例正常绒毛,15例完全性葡萄胎,5例部分性葡萄胎,14例侵蚀性葡萄胎,8例绒癌组织中MMP-7、MMP-26的表达,进行统计学分析。结果①MMP-7在正常绒毛组、葡萄胎组、侵蚀性葡萄胎组、绒癌组表达的阳性率分别为60.000%、45.000%、85.714%、100.000%,且有明显的统计学差异(x^2=11.011,P=0.012),同时,MMP-7阳性率与胎盘绒毛滋养细胞非正常增生有显著相关性(r=7.709,P=0.005),正常绒毛组、葡萄胎组分别和侵蚀性葡萄胎组、绒癌组之间阳性率比较具有明显的统计学差异(P<0.05);②MMP-26在正常绒毛组、葡萄胎组、侵蚀性葡萄胎组、绒癌组的表达阳性率分别为90.000%、45.000%、35.714%、25.000%,且有明显的差异(x^2=9.680,P=0.021),同时,MMP-26阳性率与胎盘绒毛滋养细胞非正常增生有显著相关性(r=7.519,P=0.006)。正常绒毛组、葡萄胎组、侵蚀性葡萄胎组、绒癌组MMP-26阳性率两两之间比较均有明显差异(P<0.05)。结论 MMP-7高表达MMP-26低表达可能提示妊娠滋养细胞侵袭性增强,对葡萄胎清宫组织检测MMP-7、MMP-26表达强度,有助于判断葡萄胎的侵袭性强弱。

Objective To study the expressions of MMP-7 and MMP-26 in trophoblastic disease and their relationship with trophoblastic tumor related factors, so as to provide valuable clues for early clinical diagnosis of trophoblastic disease and determination of malignant degree.Methods The protein expressions of MMP-7 and MMP-26 in 10 cases of normal villi, 15 cases of complete hydatidiform mole, 5 cases of partial hydatiform mole, 14 cases of invasive mole and 8 cases of choriocarcinama were detected using immunohistochemistry assay （ S-ABC） for statistic analysis.Results The positive rate of MMP-7 expression in normal villi group, hydatidiform mole group, invasive mole group and choriocarcinama group was 60.000%, 45.000%, 85.714% and 100.000%, respectively, and there was significant difference （χ2 =11.011, P=0.012）.Meanwhile, MMP-7 positive rate was significantly correlated with the abnormal proliferation of placental trophoblast cell （r=7.709, P=0.005）.The differences between normal villi group, mole group and invasive mole group and choriocarcinoma group were significant （P 〈0.05）.The positive rate of MMP-26 expression in normal villi group, hydatidiform mole group, invasive mole group and choriocarcinama group was 90.000%, 45.000%, 35.714%and 25.000%, respectively, and there was statistically significant difference （χ2 =9.680,P =0.021）.Meanwhile, MMP-26 positive rate was significantly correlated with the abnormal proliferation of placental trophoblast cell （r=7.519, P=0.006）.Group comparison of normal villi group, hydatidiform mole group, invasive mole group and choriocarcinama group was significantly different （P〈0.05）.Conclusion High expression of MMP-7 and low expression of MMP-26 may indicate enhanced gestational trophoblastic invasion.Detection of MMP-7 and MMP-26 expression in hydatidiform mole tissues is helpful to identify the invasive degree of hydatidiform mole.