MACC1表达与胸中段食管鳞癌侵袭、转移的关系

Investigation of expression of MACC1 and its relationship to invasion,metastasis in squamons cell carcinoma of the esophagus after esophagectomy

目的:研究结肠癌转移相关基因(MACC1)在食管鳞癌中的表达及其与淋巴结转移的关系。方法 :采用Western blot方法检测食管鳞癌细胞株和40例胸中段食管鳞癌组织及配对癌旁组织中MACC1蛋白的表达情况。结果 :KYSE510、EC109两种食管鳞癌细胞株中MACC1蛋白相对表达量分别为0.413±0.175、0.876±0.202,差异有统计学意义(P<0.05)。40例配对胸中段食管鳞癌组织中,MACC1蛋白表达水平癌组织为0.513±0.228,癌旁组织为0.248±0.179,癌组织和配对癌旁组织表达差异有统计学意义,且在伴有淋巴结转移的癌组织中表达量明显较高(P<0.05)。结论:MACC1与食管鳞癌的发生、发展及转移密切相关。

Objective：To explore the expression of colorectal carcinora metastasis associated genel（MACC） in esophageal squamous call careimma（ESCC）,and its relatonship with lymphatic metastasis. Methods：Western-blot assays were performed to detect the expression of MACC1 protein in paired sample tissues from 40 ESCC patients and cell lines. Results：The expression levels of MACC1 protein in KYSE510 and EC109 were 0.413 ± 0.175,0.876 ± 0.202,respectively. There were significant differerces in the two ESCC cell lines（P 〈 0.05）. In paired sample tissues from 40 ESCC cases,the expressions of MACC1 protein in tumor tissue specimens and peri-carcinoma tissues were 0.513 ± 0.228 and 0.248 ± 0.179,respectively. There were significant differences in the two tissues,and MACC1 expression in positive lymph mode metastasis speciemens was higher than in other speciemen（P 〈 0.05）.Conclusion：MACC1 may be closely correlated to the oncogenesis,development and metastasis of ESCC.