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PM2.5急性暴露对大鼠凝血功能的影响及其机制 被引量:3

Effects and mechanisms of short-term exposure to PM2.5 on blood coagulation function in rats
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摘要 目的探讨PM2.5暴露对大鼠凝血功能的影响及机制。方法24只雌性SD大鼠随机分为4组:对照组、PM2.53.75mg/kg组、PM257.5mg/kg组、PM2s15mg/kg组。将大鼠暴露于不同剂量PM2.5,24h后处死大鼠,取大鼠血浆检测凝血酶原时间(PT)、活化的部分凝血活酶时间(APTT)、纤维蛋白原(FIB),取BALF显微镜下细胞计数及分类,大鼠肺脏石蜡切片HE染色观察病理改变,酶联免疫吸附试验法检测BALF中IL-6、肿瘤坏死因子α(TNFd)含量、肺组织丙二醛(MDA)含量及血清血管性血友病因子(vWF)含量,蛋白免疫电泳法(Westernblot)检测血管紧张素Ⅱ的1型受体(ATl)蛋白表达。结果PM2.57.5mg/kg组、PM2.5 15mg/kg组PT、APTT值较对照组及PM2.53.75mg/kg组缩短(P〈0.05),各组血浆FIB值均无统计学差异(P〉0.05)。PM2.5各剂量组BALF中细胞总数及中性粒细胞比例均明显高于对照组(P〈0.05),且随着PM2.5剂量增加而呈剂量依赖性升高。PM2.57.5mg/kg组和PMzs15mg/kg组BALF中II-6、TNF-α含量、肺组织MDA含量、血清vwF含量及肺组织ATl受体蛋白表达明显高于对照组及PM2.53.75mg/kg组(P〈0.05)。结论PM2.5暴露可导致PT、APTT缩短,诱发血液高凝,其机制可能与炎症反应及血管内皮损伤相关。 Objective To investigate the effect of PM2 s on coagulation in rats,and further elucidate its mechanism on inflammation and endothelial injury. Methods A total of 24 rats were divided into four groups. We developed a rat model in which the animals were exposed to PM2.5 in a 24h dose-response study (3.75 mg/kg,7.5 mg/kg,and 15 mg/kg). Prothrombintime (PT),activated partial thromboplastin time (APTT),and fibringen (FIB) in plasma were detected. The total and differential cell counts in the BALF were observed. The contents of malondialdebyde (MDA), interleukin-6 (IL-6), tumor necrosis factor-a (TNF-6), and von Willebrand factor (vWF) were detected by ELISA. Western blot was performed to detect the protein level of angiotensin H type 1 receptor (AT1). Results PT and APTT in 7.5 mg/kg group and 15 mg/kg group were obviously shortened than those in control group and 3.75 mg/kg group ( P 〈 0.05). The total cell number and the percentage of neutrophils in BALF in 3.75 mg/kg group,7.5 mg/kg group,and 15 mg/kg group were obviously higher than those in control group. The contents of MDA, IL-6, TNF u,and vWF in 7.5 mg/kg group and 15 mg/kg group were obviously higher than those in control group and 3.75 mg/kg group. And the protein level of AT1 in lung of 7.5 mg/kg group and 15 mg/kg group was obviously higher than those in control group and 3.75 mg/kg group. Conclusions PT and APTT can be shortened by short-term exposure to PM2.5 , and that would lead to hypercoagulable state,which is related to inflammatory changes and endothelial damage.
出处 《国际呼吸杂志》 2014年第22期1710-1714,共5页 International Journal of Respiration
关键词 PM2.5白介素6 肿瘤坏死因子Α 丙二醛 血管性血友病因子 血管紧张素Ⅱ的1型受 凝血功能 PM2.5 Interleukin-6 Tumor necrosis factor-α Malondialdehyde Von WillebrandfactorlAngiotensin HE type 1 receptor ~Coagulation
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  • 1Brook RD, Franklin B, Cascio W, et al. Air pollution and cardiovascular disease : a statement for healthcare professionals from the Expert Panel on Population and Prevention Science of the American Heart[J]. Circulation, 2004,109 : 2655-2671.
  • 2Franehini M, Mannucci PM. Short-term effects of air pollution on cardiovascular diseases : outcomes andmeehanisms[J]. J Thromb Haemost, 2007,5:2169-2174.
  • 3Pope CA 3rd,Burnett RT,Thurston GD,et al. Cardiovascular mortality and long-term exposure to particulate air pollution: epidemiological evidence of general pathophysiological pathways of disease[J]. Circulation, 2004,109 : 71-77.
  • 4Hock G, Brunekreef B, Fischer P, et al. The association between air pollution and heart failure, arrhythmia, embolism, thrombosis, and other cardiovascular causes of death in a time series study[J].Epidemiology, 2001,12 : 355- 357.
  • 5Baccarelli A, Martinelli I, Pegoraro V, et al. Living near major traffic roads and risk of deep vein thrombosis[J]. Circulation, 2009,119:3118-3124.
  • 6Dales RE, Cakmak S, Vidal CB. Air pollution and hospitalization for venous thromboembolic disease in Chile [J]. J Thromb Haemost,2010,8:669-674.
  • 7Martinelli N,Girelli D, Cigolini D, et al. Access rate to the emergency department for venous thromboembolism in relationship with coarse and fine particulate matter air pollution[J]. PLoS One, 2012,7 : e34831.
  • 8Franchini M, Mannucci PM. Thrombogenicity and cardiovascular effects of ambient air pollution[J]. Blood, 2011,118:2405-2412.
  • 9Baccarelli A, Zanobetti A, Martinelli I, et al. Effects of exposure to air pollution on blood coagulation[J]. J Thromb Haemost, 2007,5 : 252-260.
  • 10Budinger GR, McKell JI., Urich D, et al. Particulate matter- induced lung inflammation increases systemic levels of PAI 1 and activates coagulation through distinct mechanisms[J]. PLoS One, 2011,6 : e18525.

同被引文献47

  • 1周宗灿,付立杰.现代毒理学简明教程[M].北京:军事医学科学出版社,2012:92-93.
  • 2US-EPA : http://www3, epa. gov/airquality/particlepollu- tion/health, html.
  • 3Deng F, Guo X, Liu H, et al. Effects of dust storm PM2. 5 on cell proliferation and cell cycle in human lung fibroblasts [J]. Toxicol In Vitro,2007,21 (4) : 632-638.
  • 4Farina F, Sancini G, Mantecca P, et al. The acute toxic effects of particulate matter in mouse lung are related to size and season of collection [ J ]. Toxicol. Lett, 2011,202 ( 3 ) : 209-217.
  • 5Laskin DL, Mainelis G, Turpin B J, et al. Pulmonary effects of inhaled diesel exhaust in young and old mice: a pilot project[J]. Res Rep Health Eft Inst, 2010,151: 3- 31.
  • 6Riva DR, Magalhes CB, Lopes AA, et al. Low dose of fine particulate matter (PM2. 5 ) can induce acute oxidative stress, inflammation and pulmonary impairment in healthy mice [ J ]. Inhal Toxicol, 2011,23 ( 5 ) : 257-267.
  • 7Chen T, Jia G, Wei Y, et al. Beijing ambient particle exposure accelerates atherosclerosis in ApoE knockout mice [ J ]. Toxicol Lett, 2013,223 (2) : 146-153.
  • 8Zhao H, Li W, Gao Y,et al. Exposure to particular matter increases susceptibility to respiratory Staphylococcus aureus infection in rats via reducing pulmonary natural killer cells [ J]. Toxicology, 2014,325 : 180-188.
  • 9Ying Z, Xie X, Bai Y, et al. Exposure to concentrated ambient particulate matter induces reversible increase of heart weight in spontaneously hypertensive rats [ J ]. Part Fibre Toxicol,2015,12 : 15.
  • 10Yah YH, Chou C, Wang JS, et al. Subchronic effects of inhaled ambient particulate matter on glucose homeostasis and target organ damage in a type 1 diabetic rat model [ J ]. Toxicol Appl Pharmacol,2014, 281 (2) : 211-220.

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