HPLC法测定金线草不同部位没食子酸的含量

Determination of gallic acid in different parts of Antenoron filiforme by HPLC

目的：建立金线草中没食子酸含量测定方法。方法采用 HPLC 法，色谱柱以十八烷基硅烷键合硅胶为填充剂（250 mm ×4．6 mm ，5μm），甲醇-1 mL·L-1磷酸溶液（4∶96）为流动相洗脱，检测波长272 nm ，体积流量1 mL·min^-1，柱温30℃。结果没食子酸在0．0410～0．2050μg 范围内（r ＝1）有良好的线性关系；平均回收率为101．3％，RSD 为1．7％（n ＝6）。结论该方法简便准确，为更好地开发利用金线草提供依据。

Objective To establish an HPLC method for the determination of gallic acid in different parts of A ntenoron f ili f orme . Methods The test was carried out on a C18 column（250 mm × 4 .6 mm ,5 μm） ,with methanol 1 mL· L - 1 phosphoric acid solution （4 ∶ 96） as the mobile phase .The flow rate was 1 mL · min - 1 and the detection wavelength was 272 nm ;the column temperature was 30 ℃ .Results The linear range of gallic acid was 0 .041 0-0 .205 0 μg （r = 1） ,and the average recovery was 101 .3% with RSD 1 .65% （n= 6） .Conclusion The method is simple and accurate ,and provides a basis for the further development of Antenoron f iliforme .