期刊文献+

骨髓间充质干细胞对LPS刺激BV2小胶质细胞炎症因子的影响 被引量:8

Effect of bone marrow mesenchymal stem cells on inflammatory factors released by LPS-stimulated BV-2 microglia
下载PDF
导出
摘要 目的:探讨大鼠骨髓间充质干细胞(MSCs)对LPS诱导BV2小胶质细胞炎症反应的影响。方法:采用贴壁分离法分离、纯化大鼠MSCs,BV2小胶质细胞进行常规培养传代。实验分为PBS对照组(A组)、PBS联合MSCs处理组(B组)、LPS刺激组(C组)、LPS联合MSCs干预组(D组)。BV2小胶质细胞接种于6孔板中培养过夜后,按上述实验分组处理,24 h后收集各组培养上清液用于后续实验检测。显微镜下观察BV2小胶质细胞形态变化,Griess法检测NO浓度,ELISA法检测TNF-α、IL-1β的含量。结果:MSCs可以改善活化小胶质细胞的细胞形态;C组中LPS诱导小胶质细胞活化后,培养上清NO、IL-1β、TNF-α的含量明显上升(P<0.05);D组中予MSCs处理后NO、IL-β、TNF-α释放显著减少(P<0.05)。结论:MSCs明显降低活化小胶质细胞炎症因子的释放,其可能是通过抑制小胶质细胞炎症反应而发挥神经保护作用;本研究中MSCs通过非直接接触的方式作用于小胶质细胞,推测没有进入脑内的MSCs对活化小胶质细胞亦有抑制作用。 Objective To explore the effect of bone marrow mesenchymal stem cells (MSCs) on LPS- stimulated BV2 microglia in inflammatory reaction. Methods Mouse MSCs were isolated and purified by adherence screening. The routinely cultured BV2 microglia in vitro were divided into PBS control group (group A), PBS plus MSCs treatment group (group B), LPS stimulation group (group C ) and LPS plus MSCs group (group D).MSCs and BV2 microglia were cultured in the transwell co-culture system for 24 hours. We observed BV2 mieroglia morphological changes under the microscope, detected the concentrations of NO by Griess reaction, and the level of IL-1β,TNF-α by ELISA. Results MSCs can improve the morphology of activated microglia. The concentrations of TNF-α, IL-1β and NO in culture supernatants were increased significantly (P 〈 0.05 ) after microglia activation, however, at the present of MSCs, the concentration of these inflammatory factors declined dramaticly (P 〈 0.05 ). Conclusions MSCs can significantly inhibit the activation of microglia. It may play a neuroprotective effect by reducing the inflammation of microglia. MSCs showing anti-inflammatory effects through non-direct contact with nieroglial, suggesting that MSCs outside the brain may also inhibit the activation of microglia.
出处 《实用医学杂志》 CAS 北大核心 2014年第22期3545-3548,共4页 The Journal of Practical Medicine
基金 广东省社会发展基金(编号:2011B061300026)
关键词 骨髓间充质干细胞 小胶质细胞 脂多糖 抗炎 Bone mesenchymal stem cells Microglia LPS Anti-inflammatory
  • 相关文献

参考文献12

  • 1Saijo K, Glass CK. Microglial cell origin and phenotypes inhealth and disease [J]. Nat Rev Immunol, 2011, 11 (11):775-787.
  • 2Ponomarev ED, Maresz K, Tan Y, et al. CNS-derivedinterleukin-4 is essential for the regulation of autoimmuneinflammation and induces a state of alternative activation inmicroglial cells [J]. J Neurosci, 2007, 7(40) ; 10714-10721.
  • 3刘筱,许铁.骨髓间充质干细胞免疫学特性及其移植治疗脊髓损伤[J].中国组织工程研究与临床康复,2011,15(14):2641-2644. 被引量:4
  • 4Gerdoni E, Gallo B, Casazza S, et al. Mesenchymal stem cellseffectively modulate pathogenic immune response in experimentalautoimmune encephalomyelitis [J]. Ann Neurol, 2007,61(3):219-227.
  • 5Danielyan L, Schafer R, von Ameln-Mayerhofer A, et al.Therapeutic efficacy of intranasally delivered mesenchymal stemcells in a rat model of Parkinson disease [J]. Rejuvenation Res,2011,14(1):3-16.
  • 6Lassmann H. Mechanisms of inflammation induced tissue injuryin multiple sclerosis [J]. J Neurol Sci 2008,274(1-2):45-47.
  • 7梁活,曾丽.小胶质细胞与多发性硬化[J].实用医学杂志,2010,26(5):873-875. 被引量:9
  • 8Broholm H, Andersen B, Wanscher B,et al. Nitric oxidesynthase expression and enzymatic activity in multiple sclerosis[J]. Acta Neurol Scand,2004, 109(4) : 261-269.
  • 9Baud V, Karin M. Signal transduction by tumor necrosis factorand its relatives [J]. Trends Cell Biol. 2001,11(9) :372-377.
  • 10Braddock M, Quinn A. Targeting IL-1 in inflammatory disease :new opportunities for therapeutic intervention [J]. Nat Rev DrugDiscov,2004, 3(4):330-339.

二级参考文献52

共引文献27

同被引文献54

  • 1Jongen JL, Broijl A, Sonneveld P. Chemotherapy-induced peripheral neuropathies in hematological malignancies [J]. J Neurooncol, 2015, 121(2): 229-237.
  • 2Younger J, Parkitny L, McLain D. The use of low-dose naltrexone (LDN) as a novel anti-inflammatory treatment for chronic pain[J]. Clin Rheumatol, 2014, 33(4): 451-459.
  • 3Ali I, Chugh D, Ekdahl CT. Role of 1 pathway in seizure-induced microglial activation, neurodegeneration, and neuroblast production in the adult rat brain[J]. Neurobiol Dis, 2015, 74: 194-203.
  • 4Dai JN, Zong Y, Zhong LM, et al. Gastrodin inhibits expression of inducible NO synthase, cyclooxygenase-2 and proinflammatory cytokines in cultured LPS-stimulated microglia via MAPK pathways[J]. PLoS One, 2011, 6(7): e21891.
  • 5MD S, ML M, CM A, et al. A prospective surveillance model for physical rehabilitation of women with breast cancer: chemotherapy-induced peripheral neuropathy[J]. Cancer, 2012, 118 (118): 2250-2260.
  • 6Rivera E, CianfIocca M. Overview of neuropathy associated with taxanes for the treatment of metastatic breast cancer [J]. Cancer Chemother Pharmacol, 2015, 75(4): 659-670.
  • 7Donald GK, Tobin I, Stringer J. Evaluation of acupuncture in the management of chemotherapy-induced peripheral neuropathy [J]. Acupunct Meal, 2011, 29(3): 230-233.
  • 8Jongen JL, Broijl A, Souneveld P. Chemotherapy-induced peripheral neuropathies in hematological malignancies [J]. J Neurooncol, 2015, 121(2): 229-237.
  • 9Hurley RW, Adams MC, Benzon HT. Neuropathic pain: treatment guidelines and updates[J]. Curt Opin Anaesthesiol, 2013, 26(5): 580-587.
  • 10Pachman DR, Watson JC, Lustberg MB, et al. Management options for established ehemotherapy-indueed peripheral neuropathy [J]. Support Care Cancer, 2014, 22(8): 2281-2295.

引证文献8

二级引证文献15

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部