正常人与胃病患者的口腔白色念珠菌基因型的比较

Comparison of oral Candida albicans genotype in normal and patients with gastropathy

目的观察并比较正常人群与胃病患者口腔携带白色念珠菌菌株ITS基因型的差异情况。方法对162例普通人群和104例有不适症状就诊的胃病患者进行口腔念珠菌的采样、培养并鉴定,对分离培养出的白色念珠菌菌株进行内含子转录间隔区(ITS)测序,并建立进化树比对分析序列同源性,分析两组人群口腔携带的白色念珠菌菌株ITS基因型及其进化分支情况。结果正常人群口腔标本检测出白色念珠菌36株(36/162,22.2%),胃病患者口腔分离培养出白色念珠菌38株(38/104,36.5%),经ITS序列检测,正常人群18个菌株和胃病患者17个菌株申请Gen Bank序列注册号,两组人群携带菌株建立的进化树呈各自集中趋势。结论胃病患者口腔念珠菌携带率高于正常人口腔念珠菌携带率。正常人与胃病患者口腔中分离到ITS基因型进化关系不同的念珠菌菌株,且基因型具有多态性。

Objective To observe and compare Candida albicans genotype in normal subjects and patients with gastropathy. Methods Oral Candida in 162 general population and 104 cases with uncomfortable symptoms were cultured and identified,Internal transcribed spacer region sequencing was used to analyze the homology of Candida albicans strains,by establishing the evolutionary tree. Results 36 strains of Candida albicans were isolated among162 normal oral samples（ 36 /162,22. 2%）,and 38 strains among 104 samples from patients with uncomfortable symptoms（ 38 /104,36. 5%）; ITS sequence detection,18 strains of normal group and 17 strains of patients with gastric were obtained Gen Bank sequence number,the phylogenetic tree of two groups of strains showed their central tendency respectively. Conclusion Oral Candida rate in patients with gastric is higher than that of normal human. ITS genes evolutionary branches of Candida strains isolated from normal human oral cavity and patients with gastric are different,and have genetic polymorphism.