药用地衣金刷把的质量标准研究

Studies on quality control of Lethariella cladonioides(Nyl.)Krog in medicine lichens

目的从形态结构及有效成分定性定量等方面对金刷把进行系统研究。方法通过观察和切片获得金刷把形态结构特征;以松萝酸为对照,分别用薄层色谱法和高效液相色谱法对样品中的松萝酸进行定性定量;采用硫酸-苯酚法测定总多糖和水溶性多糖含量。结果金刷把枝状体呈灌丛状,其结构为藻层分散于髓层,中央为菌丝纵向排列形成的致密中轴;6批样品总多糖平均含量为26.94%,水溶性多糖平均含量为12.51%,松萝酸平均含量为0.47%。结论研究建立的分析方法可用于金刷把药材的质量控制和评价,为规范草药市场和制定陕西地方药材标准提供参考。

Objective To research LetharieUa cladonioides （ Nyl, ） Krog Systematically based on resource, morphological characteristics and contents of active ingredients. Methods Acquiring the resource distribution and morphological characteristics of Lethariella cladonioides （Nyl.） Krog by investigation , identification and paraffin section. Usnic acid was the standard matter to establish TLC identification method and be used to establish HPLC method. The colorimetric method of phenol and sulfuric acid was used to determine the content of polysaccharides. Glucose was the standard matter to determine the content of usnic acid and polysaccharides of 6 batches of LetharieUa cladonioides （Nyl.） Krog. Results Lethariella cladonioides （Nyl.） Krog was born to the conifers dead branches on Taibai mountain at an altitude of about 3000m, branch shape body like thickets shape, the algae layer dispersed pulp layer, the density of the central axis in the centre formatted by hyphae arrange for longitudinal. 6 batches samples for the average total polysaecharide 26.94% ,water - soluble polysaccharide contents, the average level 12.51%, the average level of usnic acid 0.47%. Conclusion Tile method used quality control and evaluation of Lethariella cladonioides （ Nyl. ） Krog, and to provide reference for herbal medicine market and made local medicine standard of Shaanxi.