色谱柱极性对精油分离性能的影响（英文）

Effect of Polarity of Column on the Separation Performance of Essential Oil

采用GC-MS技术系统地研究了三种不同极性的色谱柱对新疆伊犁地区三种商品化薰衣草精油、薄荷精油和迷迭香精油的分离性能.以三种精油特征组分的理论塔板数、出峰总数和鉴定组分数量对不同极性色谱柱的分离性能进行评价.结果表明在使用单一柱型进行分析时,应针对精油成分极性分布的具体情况选择与之相对应极性的色谱柱才能实现对精油较好的分离.此外,采用两种不同极性色谱柱可以达到全面分析的目的.该研究同时也为二维色谱中柱型的选择提供了参考依据.

In this study, the effect of polarity of column on the separation performance of three essential oils was in-vestigated systematically by GC-MS. Compositions of three commercial essential oils of Lavandula angustifolia, Mentha piperita L. and Rosmarinus of cinalis L. from Yili region of Xinjiang, China were analyzed on three capillary columns with different polarities. The theoretical plate number of the characteristic components, the number of total peaks and the amount of identified components were combined to evaluate the separation performance of columns. Results showed the best separation of the oil can just be reached by a column with certain polarity according to the polarity distribution of components by a single run. And more, to reach a comprehensive analysis of oils, it was suggested to perform anal-yses on two columns with different polarities. This work also provides valuable reference for the column selection in two-dimensional gas chromatography.