紫草素对IL-22诱导HaCaT增殖及S100A7、S100A8表达的影响

Effects of shikonin on the proliferation and mRNA expressions of S100A7 and S100A8 of HaCaT cells induced by IL-22

采用WST-1法检测紫草素对白介素(IL)-22刺激永生化角质形成细胞(HaCaT)增殖的作用;实时荧光定量PCR检测IL-22刺激24、48h以及加入紫草素后HaCaT分泌S100A7、S100A8水平变化情况,探讨紫草素对IL-22刺激HaCaT增殖及分泌S100A7、S100A8的影响。结果表明:紫草素可抑制IL-22引起的HaCaT增殖;IL-22可刺激HaCaT分泌S100A7、S100A8,但该效应可被紫草素阻抑。说明紫草素对IL-22介导的体外培养角质形成细胞增殖和分泌炎症因子S100A7、S100A8有抑制作用。

WST-1 assays was used to evaluate the inhibitory effects of Shikonin on the proliferation of HaCaT cells incubated with interleukin（IL）-22. The mRNA expression of S100A7 and S100A8 of HaCaT cells was analyzed by Real- Time PCR. The effects of Shikonin on the proliferation and expressions of S100A7 and $100A8 of HaCaT cells treated with II.-22 were investigated. Results showed that the proliferation of HaCaT cells induced by IL-22 was significantly in- hibited by Shikonin; the mRNA expressions of S100A7 and S100A8 of HaCaT cells increased after treated by IL-22, but the effect could be inhibited by adding Shikonin. In vitro, Shikonin could inhibit the proliferation and the mRNA expres- sion of S100A7 and S100A8 of HaCaT cells mediated by IL-22.