摘要
以小鼠为研究对象,研究H2A.X在不同时期原始生殖细胞(PGCs)核质区的分布。结果表明:PGCs在迁移过程中,8.5、10.5、11.5dpc时H2A.X在PGCs细胞核和细胞质中呈弥散分布;13.5dpc时H2A.X在PGCs细胞核和细胞质中均有分布,但雌性小鼠PGCs中H2A.X主要集中于细胞核,而雄性主要集中于细胞质。结果提示H2A.X在PGCs中的表达可能与其性别分化后精原细胞和卵母细胞的细胞周期维持密不可分。
Mice primitive germ cells （PGCs） migration, proliferation, sex differentiation is regulated by the genome and DNA epigenetic modifications, and H2A. X is associated with DNA repair. This study aimed to explore H2A. X expression during the PGC migration process, and its relation with epigenetic modifications. Results indicated that through the PGCs single cell and the organization paraffin section immunity fluorescence method, during the migration process, with 8.5, 10.5, 11.5 dpc, H2A. X was diffusely distributed in the PGCs nucleus and cytoplasm; with 13.5 dpc, H2A. X was present in the nucleus and cytoplasm of PGCs. The female H2A. X PGCs were mainly in the nucleus, while the male were mainly concentrated in the cytoplasm. Expression, H2A. X expression in PGCs cells may be related to sex differentiation of spermatogonia and ooeytes is closely related to cell cycle maintenance.
出处
《扬州大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2014年第3期24-28,共5页
Journal of Yangzhou University：Agricultural and Life Science Edition
基金
国家重点基础研究发展计划项目(973-2007CB947403)
