番茄SlWRKY23基因的克隆及其抗病性和耐盐性分析

Cloning,Disease Resistance and Salt Tolerance Analysis of SlWRKY23 in Tomato

WRKY转录因子是普遍存在于植物界中的一类转录因子,参与了植物在生物和非生物逆境胁迫中的多种应答。前期研究表明,在PstDC3000侵染及盐胁迫下番茄SlWRKY23基因的表达水平提高。为了研究番茄SlWRKY23转录因子对PstDC3000的抗病性及在盐胁迫逆境中的功能,通过农杆菌介导的遗传转化方法获得转SlWRKY23基因植株,并对3个独立的转基因株系(WRKY23-1、WRKY23-5和WRKY23-7)进行了抗逆性分析。结果表明接种PstDC3000后,转基因植株表现明显的抗病表型,抗病防御相关基因SlPR1和SlPR1a1的表达量显著高于野生型;盐胁迫处理后,转基因植株表现出明显的抗盐表型,逆性相关基因SlRD22和SlDREB2A的表达量显著高于野生型。该结果表明SlWRKY23基因在番茄抗PstDC3000和盐胁迫过程中具有正调控作用,通过上调逆性相关基因的表达量增强抗逆性。

WRKY transcription factor is a class of transcription factor commonly exsisted in planta,which participates in various biotic and abiotc stress responses.Previous studies showed that the expression level of tomato SlWRKY23 gene was significantly up-regulated under PstDC3000 infection and salt stress.In order to study the disease resistance of tomato SlWRKY23 to PstDC3000 and its function in salt stress,transgenic SlWRKY23 plant was obtained by agrobacterium-mediated transformation and stress resistance analysis was carried out on 3 independent transgenic lines（WRKY23-1,WRKY23-5 and WRKY23-7）.The result indicated that after innoculating PstDC3000,the transgenic plant showed distinguish disease resistance,the expression quantity of disease resistance related genes SlPR1,SlPRlal was remarkably higher than that of the wild type.After treating with salt stress,the transgenic plant showed significant salt resistance,and the expression quantity of stress related genes SlRD22,SlDREB2 A was remarkably higher than that of the wild type.These results revealed that SlWRKY23 gene had positive regulatory function in tomato resistance to PstDC3000 and salt stress.It strengthened stress resistance by up-regulating the expression quantity of stress related genes.