摘要
目的 探讨病毒性脑炎(viralencephalitis,VE)患儿脑脊液(CSF)中基质金属蛋白酶-9(MMP-9)、白细胞介素-6(IL-6)及肿瘤坏死因子-α(TNF-α)水平变化的意义。方法 采用双抗夹心酶联免疫吸附法检测30例VE组患儿和16例对照组患儿CSF中神经元特异性烯醇化酶(neuron-specificenolase,NSE)、酸性钙离子结合蛋白(S100B蛋白)、MMP-9、IL-6、TNF-α 水平,并对上述指标进行相关分析。结果 VE 组患儿 CSF中NSE、S100B、MMP-9、IL-6、TNF-α水平与对照组比较均显著增高,差异均有统计学意义(P均 〈0.05);NSE与S100B之间有呈显著正相关(r=0.467,P =0.009),NSE水平与病程呈显著负相关(r= -0.472,P =0.008);MMP-9、IL-6分别与NSE、S100B呈显著正相关(r=0.698,P =0.00;r=0.559,P =0.00;r=0.812,P =0.00;r=0.664,P =0.00),TNF-α 与 S100B呈显著正相关(r=0.363,P =0.049),但与 NSE 无相关性(r=0.245,P =0.193)。结论 VE患儿神经元细胞及神经胶质细胞均有损伤,MMP-9、IL-6、TNF-α 参与 VE患儿的神经细胞病理损伤过程。
Objective To explore the significance of changes of matrix metalloproteinase-9 (MMP-9), inter- leukin-6 ( IL-6 ) , and tumor necrosis factor-alpha ( TNF-α ) protein level in the eerebrospinal fluid (CSF) of children with viral encephalitis (VE). Methods The concentration of neuron-specific enolase (NSE) , structural proteins 100B (S100B), and MMP-9, IL-6 ,TNF-α in the CSF of VE children were detected by an enzyme linked immunosorbent assay,and the correlations of them were analyzed. Results NSE,S100B,MMP-9,IL-6,TNF-α protein expression could be found significantly higher than those in the control group, and there were significant differences according to statistics expression trends( all P 〈 0. 05 ). The NSE protein expression was significantly positive related with SIOOB in the VE group ( r = 0. 467, P = 0. 009 ) , and the concentration was markedly negative related with the duration of viral encephali- tis (r = -0. 472,P = 0.008 ). MMP-9, IL-6 protein expression were significantly positive related with NSE, S100B respectively ( r = 0. 698, P = 0. 00 ; r = 0. 559,P = 0.00 ; r = 0.812, P = 0.00 ; r = 0.664, P = 0.00 ). TNF-ot protein ex- pression was positive related with CSF S100B (r = 0. 363 ,P = 0. 049) , but there was no correlation between TNF-α and NSE ( r = 0. 245, P = 0. 193 ). Conclusions The neurons and the neuroglial cells are damaged in the viral encephalitis children. MMP-9, IL-6 ,TNF-α protein may participate in the pathological damage process of nerve cells in VE children in different degrees.
出处
《中华实用儿科临床杂志》
CAS
CSCD
北大核心
2014年第22期1721-1723,共3页
Chinese Journal of Applied Clinical Pediatrics