病毒性脑炎患儿脑脊液基质金属蛋白酶-9、白细胞介素-6、肿瘤坏死因子-α变化的意义

Significance of cerebrospinal fluid matrix metalloproteinase-9, interleukin-6, and tumor necrosis factor-alpha protein in children with viral encephaliti

目的 探讨病毒性脑炎（viralencephalitis，VE）患儿脑脊液（CSF）中基质金属蛋白酶-9（MMP-9）、白细胞介素-6（IL-6）及肿瘤坏死因子-α（TNF-α）水平变化的意义。方法 采用双抗夹心酶联免疫吸附法检测30例VE组患儿和16例对照组患儿CSF中神经元特异性烯醇化酶（neuron-specificenolase，NSE）、酸性钙离子结合蛋白（S100B蛋白）、MMP-9、IL-6、TNF-α 水平，并对上述指标进行相关分析。结果 VE 组患儿 CSF中NSE、S100B、MMP-9、IL-6、TNF-α水平与对照组比较均显著增高，差异均有统计学意义（P均 〈0.05）；NSE与S100B之间有呈显著正相关（r=0.467，P =0.009），NSE水平与病程呈显著负相关（r= -0.472，P =0.008）；MMP-9、IL-6分别与NSE、S100B呈显著正相关（r=0.698，P =0.00；r=0.559，P =0.00；r=0.812，P =0.00；r=0.664，P =0.00），TNF-α 与 S100B呈显著正相关（r=0.363，P =0.049），但与 NSE 无相关性（r=0.245，P =0.193）。结论 VE患儿神经元细胞及神经胶质细胞均有损伤，MMP-9、IL-6、TNF-α 参与 VE患儿的神经细胞病理损伤过程。

Objective To explore the significance of changes of matrix metalloproteinase-9 （MMP-9）, inter- leukin-6 （ IL-6 ） , and tumor necrosis factor-alpha （ TNF-α ） protein level in the eerebrospinal fluid （CSF） of children with viral encephalitis （VE）. Methods The concentration of neuron-specific enolase （NSE） , structural proteins 100B （S100B）, and MMP-9, IL-6 ,TNF-α in the CSF of VE children were detected by an enzyme linked immunosorbent assay,and the correlations of them were analyzed. Results NSE,S100B,MMP-9,IL-6,TNF-α protein expression could be found significantly higher than those in the control group, and there were significant differences according to statistics expression trends（ all P 〈 0. 05 ）. The NSE protein expression was significantly positive related with SIOOB in the VE group （ r = 0. 467, P = 0. 009 ） , and the concentration was markedly negative related with the duration of viral encephali- tis （r = -0. 472,P = 0.008 ）. MMP-9, IL-6 protein expression were significantly positive related with NSE, S100B respectively （ r = 0. 698, P = 0. 00 ; r = 0. 559,P = 0.00 ; r = 0.812, P = 0.00 ; r = 0.664, P = 0.00 ）. TNF-ot protein ex- pression was positive related with CSF S100B （r = 0. 363 ,P = 0. 049） , but there was no correlation between TNF-α and NSE （ r = 0. 245, P = 0. 193 ）. Conclusions The neurons and the neuroglial cells are damaged in the viral encephalitis children. MMP-9, IL-6 ,TNF-α protein may participate in the pathological damage process of nerve cells in VE children in different degrees.