油酸诱导急性呼吸窘迫综合征大鼠心脏局部RAS的影响及心功能变化相关研究

Study on the relationship of cardiacal renin-angiotensin system and cardiac function in acute respiratory distress syndrome rats induced by oleic acid

目的观察油酸诱导急性呼吸窘迫综合征(ARDS)大鼠心脏局部RAS的影响及心功能的变化。方法雄性SD大鼠36只,随机分成假手术组和造模组,每组18只,各组又按造模成功后1h、2h和4h分为a、b、c 3个亚组。(1)假手术组(S组):仅进行气管切开插管和右颈总动脉穿刺置管,尾静脉注射生理盐水0.15mL/kg;(2)造模组(C组):尾静脉注射油酸0.15mL/kg,建立油酸诱导的大鼠ARDS模型。每组有效检测与活杀取材的大鼠均为18只。结果与S组比较,C组LVEDP、AngⅡ含量及Renin活性均明显升高(P<0.05),LVSP、±LVdp/dtmax明显下降。结论 ARDS可致大鼠心肌损害、心功能降低,同时激活心脏局部RAS。

Objective To observe the relationship of the cardiac function and the level of local RAS in acute respiratory distress syndrome （ARDS） rats induced by oleic acid .Methods Sprague-Daw-ley rats were randomly divided into two groups .Group S ：Control Group （only tracheal intubation ,the right carotid artery catheterization ,and tail vein injection of normal saline 0 .15 mL/kg .n=18）;Group C （ ARDS group：the ARDS model of rats induced by tail vein injection of oleic acid 0 .15 mL/kg ,n=18） .Two groups were randomly divided into 3 subgroups respectively by 1 h ,2 h and 4 h after the modeling ,and there were 6 rats in each group with effective detection and killed alive for materials .Recording the left ventricular systolic pressure （LVSP） ,maximum rising and falling rate of left intraventricular pressure （ ± LVdp/dtmax） ,and left ventricular end-diastolic pressure （LVEDP） at each time point .AngiotensinⅡ（ AngⅡ） concentration in myocardial tissue and renin activity level （ Renin ） were be detected respectively . Results In ARDS rats ,LVSP ,LVEDP ,± LVdp/dtmax ,Ang Ⅱcontent and Renin activity were high-er than control group with significant difference （P〈0 .05） .ConclusionARDS could induce myocardial damage ,decrease cardiac function and activation of cardiac local RAS in rats ..