摘要
目的:建立加味玉屏风颗粒中黄芪甲苷的含量测定方法。方法:采用高效液相色谱法,色谱柱为Diamonsil C18柱(4.6mm×250mm);流动相为乙腈-水(34:66);ELSD参数:漂移管温度105℃;载气流量2.5L/min。结果:黄芪甲苷进样量在4.821μg^24.104μg范围内与峰面积线性关系良好,回归方程为:A=1.3315C+4.7412,r=0.9992,平均回收率为99.52%,(RSD=2.42%,n=6)。结论:该方法简便、准确、重现性好,可用于加味玉屏风颗粒的质量控制。
ObjectiveTo establish a method of determining the astragaloside content in Jiawei Yupingfeng granules by HPLC- evaporating light scattering detector（ELSD）. Methods The HPLC- ELSD was used in the quantitative analysis by using the Diamonsil C18 chromatography column（250 mm × 4. 6 mm,5 μm） and acetonitril- water（34 ∶ 66） as the mobile phase. The flow rate was 1. 0 mL /min. The tube temperature of the drift tube was 105℃. The flow rate of carrier air was 2.5 L /min. Results The linear range of astragaloside reference substance was 4. 821μg - 24. 104μg,the regression equation was A = 1. 3315 C ＋ 4. 7412,r = 0. 9992. The average recovery rate was 99. 52%,RSD was 2. 42% %. Conclusion The method is strongly specific,simple to operate and accurate in the detection results,which can be used as the determination method of the astragaloside content in Jiawei yupingfeng granules.
出处
《江西化工》
2014年第3期143-145,共3页
Jiangxi Chemical Industry
