双核微核的形成机制、结局及影响因素的研究进展

Review of the origin,fate,and influencing factors of cytokinesis-block micronucleus

外周血微核试验是检测染色体损伤和快速筛选环境化学物致突变的常用遗传毒理学方法,其中胞质分裂阻滞微核试验(cytokinesis-block micronucleus assay,CBMN)是应用最广泛的检测外周血微核率的方法,后者已经作为生物标志广泛应用到染色体损伤和基因稳定性的研究中。为更好地促进CB微核在职业和环境毒物接触人群健康监护中的研究和应用,对CB微核的形成机制、结局、影响因素和优缺点及其研究进展做了综述。

Measurement of micronuclei in peripheral blood is usually used to detect chromosome damage and mutagenic action of environmental chemicals in toxicology. The cytokinesis-block micronucleus（CBMN） assay is the most widely used method for measuring micronuclei in human lymphocytes,which has been considered as a useful biomarker to detect chromosome damage and gene stability. In this paper,the formation mechanism,endpoint,and the influencing factors of CBMN and the advantage and disadvantage of CBMN assay is reviewed to boost the use of cytokinesis-block micronucleus in protecting workers exposed to environmental chemicals.