叶绿体信号通过诱导抗氧化酶和花青素参与拟南芥环境胁迫响应

Plastidsignals in Arabidopsis response to environmental stress through inducing synthesis of antioxidant enzymes and anthocyanins

本文研究了渗透胁迫和冷胁迫对于叶绿体信号缺失突变体gun1、gun5和abi4的影响.实验结果表明,叶绿体信号缺失突变体对胁迫的抵抗力下降,表现在植株含水量降低,电导率和丙二醛含量升高.并且发现叶绿体信号缺失突变体中抗氧化酶如POD、SOD、APX活性明显下降,活性氧(超氧离子)积累明显加重,此外叶绿体信号缺失突变体不能诱导植物体内花青素的合成.本文初步探讨了叶绿体信号与植物体内活性氧和花青素合成途径的关系,表明叶绿体信号可以通过诱导抗氧化酶和花青素从而抵抗环境胁迫.

The influence of osmotic stress and cold stress were measured on gun1, gunSand abi4 mutants with plastid-signaling deficient. Results suggested that the resistance of mutants with plastid-signaling deficient decreased significantly along with relative water content （RWC）, electric leakage （EL） and ma- lonaldehyde （MDA） content increasing. The antioxidant activity of enzyme such as SOD, POD and APX were very low in mutants that resulted in the massive accumulation of ROS （super-oxygen ion）, and at the same time mutants with plastid-signaling deficient could not induce the accumulation of anthocya- nins. This study preliminary analysed the relationship between plastid signals and ROS and anthocya- nin＇s synthesis process in plant which suggested that plastid signals in Arabidopsis responsed to envi-ronmental stress through inducing synthesis of antioxidant enzymes and anthocyanins.