汉防己甲素联合维生素E对急性百草枯中毒的实验治疗研究

Experimental study on therapeutic efficacy of tetrandrine combined with Vitamin E against acute toxicity of paraquat

目的探讨汉防己甲素(TET)和维生素E(VE)对急性百草枯(PQ)中毒的治疗作用。方法大鼠腹腔注射百草枯15 mg/kg染毒后,每天灌胃TET(30 mg/kg)、VE(45 mg/kg),联合治疗组按上述两种药物的给药时间和剂量同时治疗;未治疗组给等体积的生理盐水。观察每组动物的中毒表现和肺组织结构改变。结果各组大鼠中毒症状相似;治疗组大鼠肺损伤积分均明显低于染毒组,且差异有统计学意义(P<0.05或P<0.001),尤以联合治疗组为著。病理观察可见PQ中毒后肺泡壁充血和炎症细胞浸润,治疗组水肿、充血、炎细胞浸润及肺纤维化减轻。结论 TET和VE联合治疗可拮抗PQ中毒所致的肺纤维化。

Objective To investigate the therapeutic efficacy of tetrandrine（TET） and Vitamin E（VE） against acute poisoning by paraquat（PQ）. Methods Paraquat was given to rats by intraperitoneally injected,the treated rats were given TET、VE or TET plus VE per day by intragastric administration,the control rats were injected intraperitoneally with same volum of saline solution,then observe the toxic manifestations and histological changes of the lungs. Results The results showed that the toxic manifestations were quite similar among the toxic groups,but the pathological scores of pulmonary fibrosis in treatment groups were significantly lower than those of toxic group,especially the combined therapeutic group. The pathological examination found the principal changes in lungs caused by PQ were alveolar congestion and leukocyte infiltration,while all the changes such as edema,congestion,leukocyte infiltration and pulmonary fibrosis etc. seemed alleviated. Conclusion The study suggested that TET combined with VE has some tagonistic effects on pulmonary damage such as fibrosis caused by acute paraquat poisoning.