艾灸足三里、肾俞穴对骨性关节炎模型大鼠PGE2、NO及IL-1β的影响

Effect of Moxibustion at Zusanli and Shenshu Points on PGE2,NO and IL-βin OA Model Rats

观察艾灸足三里、肾俞穴对骨性关节炎(OA)模型大鼠关节液中前列腺素E2(PGE2)、一氧化氮(NO)及血清中白细胞介素-1β(IL-1β)水平的影响,进一步研究艾灸足三里、肾俞穴干预OA炎症发生发展的作用机制。将24只SPF级健康雌性SD大鼠随机分为Sham组、对照组和艾灸组。通过切除双侧卵巢并切断右侧膝交叉及内侧副韧带建立OA动物模型,术后第4周,艾灸组给予艾灸足三里和肾俞穴治疗,Sham组及模型灌服2ml的生理盐水,分别于术后第4、8周采用酶联免疫吸附法(ELISA)法测定关节液中PGE2、NO及血清中IL-1β的水平,对比各组数据。术后第8周,艾灸组关节液中的PGE2、NO较Sham组明显升高(P<0.01),较对照组明显减少(P<0.05),较Sham组明显升高(P<0.01),艾灸组血清中IL-1β的水平较对照组明显减弱(P<0.01);艾灸组关节液中的PGE2较术后第4周明显减少(P<0.05),其关节液中的NO及血清中IL-1β较术后第4周明显减少(P<0.01)。艾灸足三里、肾俞穴能明显抑制OA大鼠关节液中PGE2、NO及血清中的IL-1β水平,减缓OA炎症的发生发展,可作为OA的有效治疗方法。

To observe the effects of moxibustion at Zusanli and Shenshu points on the levels of PGE2 and NO of the synovial fluid and IL-βof the serum in OA model rats,and study on the mechanism of moxibus-tion at Zusanli and Shenshu points to intervene the development of OA inflammation,twenty-four SD rats were randomly divided into a sham operation group,a control group and a treatment group.The rats in the sham operation and the control groups were treated with 2 mL normal saline everyday,and the rats in treatment group were treated with moxibustion at Zusanli and Shenshu points in 4 weeks after operation. ELISA was used to determine the contents of PGE2 and NO of the synovial fluid and the IL-1βof the ser-um in 4 and 8 weeks after operation.The results showed that in the 8 weeks after operation ,PGE2 and NO of the synovial fluid significantly increased in the treatment group than that of sham group （P 〈0.01）,while it decreased in the control group （P 〈 0.05）.IL-1βof the serum decreased significantly in the treatment group than that of control group （P 〈 0.01）;Compared with the ones in the 4 weeks after oper-ation,the PGE2 of the synovial fluid decreased significantly（P 〈 0.05）;while the NO of the synovial fluid and the IL-1βof the serum decreased significantly（P 〈 0.01）.Moxibustion at Zusanli and Shenshu points can obviously inhibit the expression of PGE2 and NO of the synovial fluid and IL-β of the serum of OA model rat,slow the evolutive inflammation of osteoarthritis.