DNMT1、PDCD4在舌鳞状细胞癌中的表达及临床意义

Expression and clinical significance of DNMT1 and PDCD4 in tongue squamous cell carcinoma

目的探讨DNA甲基转移酶1(DNMT1)、程序性细胞死亡因子4(PDCD4)在舌鳞状细胞癌(TSCC)组织中的表达及临床意义。方法采用免疫组织化学Elivision二步法检测40例TSCC组织及其相对应的癌旁非肿瘤组织中DNMT1、PDCD4蛋白表达水平,并分析两者蛋白表达与临床病理参数的关系。结果在TSCC组织中DNMT1蛋白呈高表达,而PDCD4蛋白呈低表达甚至不表达;两者在癌旁非肿瘤组织中的表达则与之相反。两者的表达呈明显的负相关(r=-0.452,P<0.05)。DNMT1蛋白表达与组织分化类型、有无淋巴结转移及TNM临床分期相关(P<0.05),与性别、年龄无关;而PDCD4蛋白的表达则只与区域淋巴结有无转移和TNM分期有关,与性别、年龄及组织病理分化程度无关。结论提示DNMT1、PDCD4蛋白异常表达与TSCC的发生、发展密切相关,DNMT1可能通过负向调节PDCD4的蛋白表达使抑癌基因沉默或消失而致肿瘤发生。

Objective To investigate the expression of DNM T1 and PDCD4 in Tongue Squamous cell carcinoma and their signif‐icance to Clinical .Methods The protein expression of DNM T1 and PDCD4 in 40 cases of TSCC tissues and The adjacent no tumor tissues were measured by Elivision Two Step immunohistochemical method（IHC） ,the relationship between DNM T1 ,PDCD4 and clinicopathological parameters were analyzed .Results There was positively over expression of DNM T1 while the expression of PD‐CD4 was at a low lever or lost in TSCC tissues ,the expression of two genes DNM T1 and PDCD4 in the adjacent no tumor tissues were in contrast ;there was a significant negative correlation between DNM T1 and PDCD4 expression in TSCC（r = - 0 .452 ,P〈0 .05） .The expression of DNM T1 protein were associated with histopathological differentiation types ,regional lymph node metasta‐sis and TNM staging（P〈 0 .05） ,it had nothing to do with age and gender ;the expression of PDCD4 protein were associated with regional lymph node metastasis and TNM staging（P〈 0 .05） ,and it had nothing to do with age ,gender ,histopathological differenti‐ation types .Conclusion Implying the abnormal expression of DNM T1 and PDCD4 protein might be closely related to the develop‐ment and progression of TSCC .DNM T1 may be participates in the inactivation of PDCD4 expression ,and led to the development of the cancer at last .