杜仲药材有效成分与环境因子的灰色关联度分析

Grey Correlation Degree Analysis Between Active Ingredients in Barks or Leaves of Eucommiae Cortex and Environmental Factors in Their Habitats

目的：运用灰色关联度法分析影响杜仲皮和叶中4种有效成分含量产地差异性的主要环境因子。方法：利用HPLC同时测定10个产地10年生杜仲皮和叶中桃叶珊瑚苷、京尼平苷酸、绿原酸、松脂醇二葡萄糖苷的含量，流动相分别为乙腈（A）-0.1％磷酸水（B）梯度洗脱（0～25min，5％～13％A；25～45min，13％～15％A；45～80min，15％～21％A），流动相乙腈（A）-0．1％磷酸水（B）梯度洗脱（0～20min，5％～12％A；20～35min，12％～13％A；35～45min，13％-19％A；45～65min，19％～25％A），检测波长均为210nm。检测杜仲生长地土壤的全氮、全磷、全钾、碱解氮、速效磷、速效钾、有机质及pH，获得土壤因子数据，运用灰色关联度法对4种有效成分与气候及土壤因子的相关性进行分析。结果：10个产地同一生长年限杜仲皮、叶中4种有效成分的含量存在较大差异。碱解氮、速效钾、有机质、年平均相对湿度、年平均最高气温对4种成分在杜仲不同组织中的影响较大。结论：杜仲不同组织部位中4种有效成分含量与环境因子的灰色关联度分析明确了主导环境因子，为合理施肥及环境调控来提高杜仲次生代谢产物的含量提供参考，为杜仲的适宜种植区选择及其道地性产生原因分析提供实验依据。

Objective： To find out dominant environmental factors which impact main effective components in barks or leaves of Eucommiae Cortex by gray correlation analysis. Method： HPLC was employed to determine contents of geniposidic acid, aucubin, pinoresinol diglucoside and chlorogenic acid at the same time in barks or leaves of Eucommiae Cortex at 10 years from ten habitats, detection wavelength were 210 nm. Soil physieochemical characteristics were detected, such as total nitrogen, total phosphorus, total potassium and so on, in order to obtain soil factor data, data were analyzed by grey correlation method. Result： There were big differences between barks or leaves of Eucommiae Cortex at the same growth ages in ten habitats. Effects of alkali- hydrolyzable nitrogen, rapidly available potassium, organic matter, the annual average relative humidity, the annual average maximum temperature on contents of four compounds from different tissue were remarkable. Conclusion： Grey correlation analysis finds out dominant environmental factors that impact contents of active ingredients in different tissues of Eucommiae Cortex. It may provide a clue to improve content of secondary metabolites in Eucommiae Cortex by rational application of fertilizer and environmental control, it also provides scientific guidance for choosing suitable areas and analyzing genuineness causes of Eucommiae Cortex.