HPLC法测定当归黄精膏中阿魏酸的含量

Determination of Ferulic Acid in Danggui Huangjing Decoction by HPLC

目的:建立测定定当归黄精膏中阿魏酸含量的检测方法。方法:采用HPLCI法,色谱柱:FORTIS-C18柱(4.6 mm×250 mm,5μm),以乙腈:0.05%磷酸溶液(17∶83)为流动相,流速:1.0 m L·min-1,柱温:30℃,检测波长:316 nm。结果:阿魏酸在0.007-0.140 mg/m L浓度范围内有良好线性关系(r=0.9998),平均加样回收率为97.5%,RSD%为1.54%。结论:本方法灵敏度高、准确、重复性好、专属性强,可作为控制当归黄精膏质量的重要方法。

Objective： To establish a HPLC method for the determination of ferulic acid in Danggui Huangjing decoction（当归黄精膏）. Methods： The HPLC system consisted of the FORTIS-Cls（4.6 mm×250 mm, 5μm）column, the mobile phase consisted of acetonitrile（ 17）-0.05% H3PO4（83）, the flow rate was 1.0 mL·min^-1, the column temperature was 30 %, The UV detector was set at 316nm. Results： The linear response range was 0.007-0.140 mg/mL （r=0.9998）. The average recovery of ferulic acid was 97.5%, and RSD was 1.54%. Conclusions： The method high sensitivity, accurate, repeatable and high secificity. It can be an important method to control the standard of Danggui Huangjing decoction.