红球菌R04气囊蛋白GvpA与GvpJ表达与定位的初步研究

Expression and localization of gas vesicle protein GvpA and GvpJ in Rhodococcus sp.R04

目的探讨红球菌R04气囊蛋白GvpA与GvpJ表达与定位的初步研究。方法通过气囊结构蛋白GvpA与GvpJ基因的克隆和鉴定,分析蛋白的诱导表达,以及荧光显微镜观察。结果由于蛋白质构成的气囊结构广泛存在于自然界许多微生物中,其内充满气体,为水生微生物提供浮力。基因组测序发现土壤微生物红球菌Rhodococcus sp.R04中也存在气囊蛋白基因簇。经比对推测,在红球菌中GvpA是构成气囊的主要结构蛋白,GvpJ与GvpA同源性很高,推测其参与气囊帽子结构的形成。结论本实验克隆并表达了红球菌R04气囊蛋白GvpA和GvpJ,并对其蛋白定位进行了分析,该研究为阐明R04气囊蛋白的生理功能提供了基础。

Objective To conduct a preliminary study on the expression and location of airbag protein GvpA and GvpJ in Rhodococcus sp.R04.Methods Through the airbag structure protein GvpA and GvpJ gene cloning and identification,the protein induced expression was analyzed and fluorescence microscope observation was performed.Results Due to the airbag structure of the protein components widely exists in many microorganisms in nature,which is full of gas,provide buoyancy to aquatic organisms.The genome sequencing found that the airbag protein gene cluster also exists in soil microbial Rhodococcus sp.R04.By comparing speculation,GvpA in Rhodococcus sp.R04 is the main structural protein constituting airbags,GvpJ and GvpA have very high homology,speculating that GvpJ and GvpA participate in the formation of airbag cap structure.Conclusion This experiment clones and expresses the GvpA and GvpJ R04 airbag protein of Rhodococcus sp R04,and its protein localization is analyzed,the research provides the basis for elucidating the physiological function of R04 airbag protein.