多种炎性疾病患者外周血CD4^+和CD8^+T淋巴细胞的检测与临床意义分析

Detection of peripheral blood of CD4+and CD8+T lymphocytes in multiple inflammatory diseases and its clinical significance

目的探讨恶性肿瘤、肺结核、干燥综合征、葡萄膜炎等多种炎性疾病患者外周血CD4+和CD8+淋巴细胞的变化及其临床意义。方法应用BD FACSCanto II流式细胞仪检测上述疾病患者外周血CD4+、CD8+T细胞并计算两者比值,同时检测健康者标本20例作为健康对照组,进行统计分析。结果与健康对照组比,恶性肿瘤组CD4+T淋巴细胞、CD4+/CD8+均明显降低,CD8+T淋巴细胞增高,差异有统计学意义(P<0.05);全身炎性反应综合征组CD4+T淋巴细胞、CD4+/CD8+均明显降低,CD8+T淋巴细胞增高,差异有统计学意义(P<0.05);葡萄膜炎组CD8+T淋巴细胞增高,差异有统计学意义(P<0.05);肺结核组CD4+T淋巴细胞降低,差异有统计学意义(P<0.05)。结论患者的CD4+、CD8+T淋巴细胞异常,表明机体细胞免疫功能异常。流式细胞仪检测CD4+、CD8+T淋巴细胞可以作为患者临床免疫功能的初步诊断指标,辅助疾病治疗和病情观察。

Objective To investigate the change of peripheral blood CD4^＋and CD8^＋T lymphocytes and its clinical significance in the patients with multiple inflammatory diseases such as malignant tumors,tuberculosis,Siogren syndrome,uveitis and systemic inflammatory response syndrome（SIRS）.Methods The peripheral blood CD4^＋,CD8^＋T lymphocytes in the patients with above diseases were detected by the BD FACSCanto II flow cyctometry and the ratio of CD4^＋/CD8^＋T cells was calculated.At the same time 20 individuals with healthy physical examination as the healthy control group were measured.The detection results were statistically analyzed.Results Compared with the healthy control group,peripheral blood CD4^＋T lymphocytes and the ratio of CD4^＋/CD8^＋in the malignant tumors group were significantly decreased with statistical difference between the two groups（P〈0.05）;peripheral blood CD4＋T lymphocytes and the ratio of CD4^＋/CD8^＋in the SIRS group were significantly decreased and CD8^＋T lymphocytes were increased compared with the healthy control group,the difference had statistical significance between the two groups（P〈0.05）;CD8^＋T lymphocytes in the uveitis group were increased compared with the healthy normal controls,the difference had statistical significance（P〈0.05）;CD4^＋T lymphocytes in the pulmonary tuberculosis group were decreased,the difference had statistical significance（P〈0.05）.Conclusion The abnormality of peripheral blood CD4^＋,CD8^＋T lymphocytes in the patients means the damage of cellular immune function.The CD4^＋and CD8^＋T lymphocytes detected by flow cytometry can be considered as the preliminary diagnostic indicators of the immune function,which can assist the disease treatment and condition observation.