布鲁菌病患者外周血NK细胞和T淋巴细胞亚群的变化

Changed of NK cell and T-lymphocyte subsets in peripheral blood of patients with brucellosis

目的了解布鲁菌病患者性别、年龄、职业分布特征及并发症,外周血NK细胞和T淋巴细胞亚群的变化及意义。方法对101例布鲁菌病患者采用流行病学调查法进行调查,采用流式细胞仪抗体双表法检测38例布鲁菌病和非布鲁菌病发热患者外周血NK细胞(CD16+CD56+)、淋巴细胞绝对值(CD45+)、T淋巴细胞绝对值(CD3+)、T辅助细胞绝对值(CD3+CD4+)、T抑制/细胞毒细胞绝对值(CD3+CD8+)、B淋巴细胞绝对值(CD19+)、CD4/CD8比值,并进行分析。结果布鲁菌病患者以男性居多,中老年患者居多,发病人群主要为农牧民及从事防疫工作者,并发症以肝功能损害最多;布鲁菌病患者外周血NK绝对值显著低于非布鲁菌病发热患者组,具有统计学意义(t=-2.58,P<0.05)。结论布鲁菌病以男性中老年患者居多,以农牧民和防疫员为主,布鲁菌病患者外周血NK细胞常受损伤。

Objective To investigate the characteristics of gender, age, occupation distributions and complication of patients with brucellosis, explore the changes of NK cell and T-lymphocyte subsets, and the related significance. Methods Total of 101 cases of brucellosis were analyzed by descriptive epidemiological study. NK and T/B-lymphocyte subsets were studied in peripheral blood of 38 patients with brucellosis and 35 cases with non-brucellosis fever. Results The majority of cases were male elderly patients, mainly occurred among farmers, herdsmen and stuff engaged in the epidemic prevention. The complication of brucellosis was priority to liver damage. The number of NK cell in patients with brucellosis was below compared with in patients with non-brucellosis fever, with significant differeces（t =-2.58, P 〈0.05）. Conclusions The majority of patients with brucellosis were male elderly person. Outbreak mainly occur in farmers, herdsmen and stuffs engared in epidemic prevention field. Brucellosis always damage the NK cell of peripheral blood.