miR-143在HCC组织中的表达及其临床意义

Expression and clinical signifi cance of micro R-143 in hepatocellular carcinoma tissue

目的通过检测肝细胞肝癌(HCC)组织中micro RNA-143(mi R-143)的表达,探讨mi R-143在HCC侵袭转移过程中的作用。方法收集45例HCC组织和对应的癌旁肝组织标本,采用实时荧光定量PCR(q PCR)法检测上述组织中mi R-143的表达,并分析其与HCC的临床病理学参数及HBV的关系。结果癌旁肝组织中无mi R-143表达的病例,HCC组织中mi R-143的阳性表达率为88.89%(40/45)。mi R-143的表达与肿瘤数目、肿瘤包膜、门静脉癌栓、癌细胞分化、远处转移和肿瘤分期呈显著相关(P均<0.05),而与患者年龄、性别、肿瘤直径和HBV感染无关(P均>0.05);mi R-143表达阳性与阴性的HCC患者3年生存率分别为89.12%、32.56%,差异有统计学意义(P<0.005)。结论 mi R-143表达可能与HCC的侵袭转移有关,mi R-143可作为预测HCC预后的标志物。

Objective To explore the possible function and significance of miR-143 expression by detecting the miR- 143 expression in carcinoma and the corresponding para-carcinoma tissue from patients with hepatocellular carcinoma. Methods Total of 45 samples of HCC and the corresponding para-carcinoma tissues were collected from our hospital, and the real-time quantitative reverse transcription （qRT-PCR） was used to test the miR- 143 expression. The correlation between the miR-143 expression and the clinical pathological parameters of HCC was analyzed. Results There was no miR-143 expression in the corresponding para-carcinoma tissues, and the positive expressing rate ofmiR-143 in HCC tissues was 88.89% （40/45）. It＇s been showed positive correlation between the miR-143 expression and the tumor number, tumor capsule, portal vein tumor thrombus, tumor differentiation grade, distant metastasis and tumor stage （all P 〈 0.05）, but no correlation between the miR-143 expression and the age, sexuality, tumor diameter, HI3V infection （all P 〉 0.05）. MiR-143 expression of positive and negative of HCC patients with 3 year survival rates were 89.12%, 32.56%, the difference was statistically significant （P 〈 0.005）. Conclusions miR-143 may contribute to the invasion and metastasis of HCC and was an biological marker for predicting the prognosis of HCC.