丹瓜方对转基因糖尿病小鼠心脏损伤的作用

Study of Dan Gua-Fang in Preventention and Treatment of Heart Injury of Genetically Induced Diabetic Mice

探讨丹瓜方对转基因糖尿病小鼠心脏组织的影响。32只ApoE-/-小鼠数字随机等分为模型组、丹瓜方组、联合用药组(丹瓜方+吡格列酮)、吡格列酮组。喂以普通饲料,各组分别给药12周。小鼠处死,取心脏、匀浆,免疫组化测心脏血清反应因子(SRF)含量;解剖冠状动脉,光镜观察比较。结果:与模型组比较,联合用药组与丹瓜方组SRF阳性率降低有显著性差异(P<0.01);形态学观察,丹瓜方组与联合用药组冠状动脉内膜大部分无增厚。结果表明:丹瓜方对糖尿病小鼠心脏具有保护作用。

To observe the effect of D an Gua-Fang on the heart of genetically induced diabetic mice. T hirty-tw o A poE-/-diabetic m ice w ere random ly divided into the m odel group, D an-gua prescription group, the com bined treatm ent group （Dan-gua prescription＋ pioglitazone） and pioglitazone group. E ach group w as given m edicine respectively, and fed w ith norm al diet for 12 w eeks. T he heart hom ogenates production w as used to m easure related indicators. T he expression of SR F in heart hom ogenates w as detected by im m unohistochem ical. T he C oronary w as observed under light m icroscopy. C om pared w ith the m odel group, there w ere significant difference of SR F positive rate betw een com bination group and D an-gua prescription group （P＆lt;0.01）. T he m orphology of D an-gua prescription group and the com bination group w as significantly im proved： m ost of the tunica intim a of the coronary artery cannot be found thickening. C om pared w ith the control group, Pioglitazone group＆#39;s effect is w eaker,butbetterthan thatofthe untreated group.D an G ua-Fang hasprotective effecton the heartofdiabetic m ice.