福建省长乐市80株结核分枝杆菌临床分离株可变数目串联重复序列基因分型研究

Multiple-locus variable number tandem repeat analysis on genotypes of 80 strains of Mycobacterium tuberculosis in Changle,Fujian province

目的研究福建省长乐市结核分枝杆菌临床分离株的基因型构成、主要流行株及其相关传播流行特征。方法选择15个可变数目串联重复序列(variable number of tandem repeats,VNTR),对来源于长乐市结核分枝杆菌的临床分离菌株DNA进行检测,检测结果使用Bio Numerics 5.0软件进行聚类分析。结果 80株结核分枝杆菌被分为Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ、Ⅶ、Ⅷ7大基因群,以Ⅰ群为主,包含59(73.8%)个株菌;流动人口结核分枝杆菌基因群为Ⅰ、Ⅱ、Ⅳ、Ⅶ、Ⅷ,常住人口为Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ、Ⅷ;流动人口与常住人口的结核分枝杆菌VNTR基因型存在一定差异,但均以Ⅰ群为主。Ⅰ群菌株耐药性与其他基因群的耐药性差异无显著统计学意义(P>0.05)。结论初步明确长乐市结核分枝杆菌菌株有7大基因群,存在明显的基因多态性,以Ⅰ群流行为主,应加强此群菌株流行的监控。

Objective To understand the genotype distribution of the clinical isolates of Mycobacterium tuberculosis, major circulating strains and related circulating patterns in Changle, Fujian province. Methods Fifteen locus of variable number tandem repeats （VNTR） were used to detect 80 clinical strains of M. tuberculosis isolated in Changle, and clustering analysis of the strains were performed by using BioNumerics 5.0. Results The 80 M. tuberculosis strains were classified into 7 gene groups, i.e. Ⅰ,Ⅱ,Ⅲ,Ⅳ,Ⅴ,Ⅶ and Ⅷ. Group Ⅰ was the major group which had 59 strains（73.8% ）. The gene groups of M. tuberculosis were Ⅰ ,Ⅱ, Ⅳ,Ⅴ and Ⅵ were detected in floating population and Ⅰ , Ⅱ , Ⅲ, Ⅳ, Ⅴ and Ⅷ were detected in local population. The VNTR analysis indicated that some differences existed in the genotypes of M. tuberculosis detected between floating population and local population, but gene group I was predominant in two populations. No significant difference was detected in drug resistance between the isolates in group Ⅰ and other groups （ P 〉 0. 05 ）. Conclusion Seven gene groups of M. tuberculosis were detected and obvious gene polymorphism was observed in Changle. Group Ⅰ was the predominant genotype in both floating population and local population. It is necessary to strengthen the surveillance for the circulating of the strains in this gene group.