浙江省永嘉县人感染H7N9禽流感外环境监测分析

Surveillance for avian influenza A(H7N9) virus in outer environment in Yongjia 325102,Zhejiang

目的了解外环境中H7N9病毒分布情况,为防控H7N9禽流感工作提供科学依据。方法采集活禽养殖场、农贸交易市场、宰杀场和农家酒店等鸡(鸭)的咽喉分泌物、肛粪、血、翅膀涂抹物、蛋涂抹物、鸡、鸭宿涂抹物、下蛋槽涂抹物和房水等标本,用上海之江生物科技股份有限公司生产的禽流感病毒(2013)核酸测定试剂盒(实时荧光定量聚合酶链反应)进行H7N9禽流感病毒检测,用Excel软件进行数据统计分析。结果监测8个月中,有4个月出现H7N9禽流感病毒阳性,阳性出现率50.00%;68个场所中7个检出H7N9禽流感病毒阳性,阳性率为10.29%(7/68),阳性场所波及县内多个不同区域,相互间隔距离较远。检测标本1670份,其中鸡相关标本1138份,阳性23份,阳性率2.02%(23/1138),鸭相关标本532份,未见阳性。活禽养殖场15所,阳性3所(3/15),623份鸡标本,阳性14份,阳性率2.25%(14/623);农贸交易市场38个,阳性4个,阳性率10.53%(4/38),355份鸡标本,阳性9份,阳性率2.54%(9/355)。携带H7N9禽流感病毒的活禽密切接触者26人,咽拭子病毒检测结果均阴性,未出现感染病例。结论环境场所存在H7N9禽流感病毒,该病毒在鸡群中存在的概率明显高于鸭群。

Objective To understand the distribution of avian influenza A （H7N9）virus in outer environment in Yongjia, Zhejiang province and provide evidence for the prevention and control of human infection with H7N9 virus. Methods The samples of chicken or duck throat secreta, anus fecess, blood, wing smear, egg smear, cage smear, egg trough smear and water were collected from poultry farms, farm produce markets, slaughter houses and rural restaurants to detect H7N9 virus by using nucleic acid detection reagent kit produced by Shanghai Zj Bio-Tech Co. Ltd. Excel software was used to analyze the results statistically. Results During the 8 months surveillance, H7N9 viruses were detected in 4 months（50% ）, and the viruses were detected in 7 of 68 surveillance sites（ 10. 29% ）. The surveillance sites were far from each other. A total of 1670 samples were detected, including 1138 chicken related samples （23 were positive, accounting for 2. 02% ）and 532 duck related samples（ none was positive）. H7N9 viruses were detected in 3 of 15 poultry farms（20% ）, among the 623 chicken samples, 14 were positive（2. 25% ）. And H7N9 viruses were detected in 4 of 38 farm produce markets（ 10. 53% ）, among the 355 chicken samples, 9 were positive（2. 54% ）, All the 26 persons with close contacts with H7N9 positive alive poultry were negative in testing of their throat swabs and no human infections with H7N9 virus were detected. Conclusion H7N9 virus circulated in outer environment. The probability of circulation of H7N9 virus in chickens was higher than that in ducks, indicating that the virus is prone to spread in chickens. The pathogenicity of H7N9 is low to humans and the transmissibility is weak.