摘要
丙型病毒性肝炎(丙肝)病毒感染呈世界性传播,严重威胁人类健康。预防和控制丙肝传播的重要手段之一是临床早期明确诊断,及时抗病毒治疗。临床常用的实验诊断血清标志物主要有HCV-Ab、HCV-Ag,以及HCVRNA定性/定量、HCV-基因型及基因亚型的检测。本文介绍了丙肝病毒感染的实验诊断技术及方法学(包括ELISA、RIBA、CLIA、DIFA、real-time PCR和RT-LAMP)研究的现况,希望能够为丙肝的临床诊断、疾病监测及其流行病学调查有所裨益。
Hepatitis C virus infection spread worldwide, posing a serious threat to human health. One of the important means to prevent and control the spread of hepatitis C is to conduct early diagnosis and give prompt antiviral therapy. Commonly used serum markers in clinical laboratory diagnosis of hepatitis C are HCV-Ab, HCV-Ag, and major tests included qualitative/quantitative HCV-RNA detection and HCV genotype and sub genotype detection. This paper summarizes the current status of the research on technology and methodology of laboratory detection of hepatitis C virus, including ELISA, RIBA, CLIA, DIFA, real-time PCR and RT-LAMP, for the purpose of facilitating the clinical diagnosis, surveillance and epidemiological survey of hepatitis C.
出处
《疾病监测》
CAS
2014年第10期837-844,共8页
Disease Surveillance
