HLPC梯度洗脱测定抗荨胶囊中升麻素苷和5-O-甲基维斯阿米醇苷的总含量

Quantitative Determination of Cimicifugoside and 5-O-omethylvisammios-De in Anti-nettle Capsules by HPLC Gradient Elution

目的：建立抗荨胶囊（防风蒺藜蝉蜕黄芪当归白鲜皮等）中升麻素苷和5-O-甲基维斯阿米醇苷含量的高效液相色谱的方法。方法：以WondaSil C18-WR（4.6 mm×150mm,5μm PH 1-10）为色谱柱;流动相采用甲醇（A）-0.05%磷酸溶液（B）梯度洗脱：0-6min,40%B,6-17min,45%（B）,17-30min,75%（B）,升麻素苷和5-O-甲基维斯阿米醇苷检测波长为254nm,流速为1.0mL·min-1,柱温为30℃,进样量为10μl。结果：用梯度洗脱得到了较好的分离效果,升麻素苷18.4-92.0μg/mL之间线性关系良好,r=0.9999550（n=6）;5-O-甲基维斯阿米醇苷在22.0-110.0μg/mL之间线性关系良好,r=0.9996565（n=6）,平均回收率分别为100.28%和100.56%。结论：该研究同时进行升麻素苷、5-O-甲基维斯阿米醇苷的定量分析,方法专属性强,准确度高,可用于抗荨胶囊中升麻素苷和5-O-甲基维斯阿米醇苷的含量测定。

Objective： To establish a high-performance liquid chromatography（HPLC） for simultaneous determination of cimicifugoside and 5-O-methylvisammios-De in anti-nettle capsules（radix saposhnikoviae,caltrop,periostracum cicadae,Astragalus membranaceus,angelica sinensis,dictamnus dasycarpus,etc.）. Methods： WondaSil C18-WR（4. 6 mm × 150 mm,5 μm PH 1- 10） is used as the column; Mobile phase has been made gradient elution with（A）- 0. 05% phosphoric acid： 0 - 6min,40% B,6 - 17 min,45%（B）,17- 30 min,75%（B）,the detection wavelength of cimicifugoside and 5-O-methylvisammios-De is 254 nm,the flow speed is 1. 0mL·min- 1,the column temperature is 30℃,and the injection volume was 10 μl. Results： A better separation result is obtained by the gradient elution. There was a good linear relationship for cimicifugoside from 18. 4 to 92. 0 μg /mL r = 0. 9999550（n = 6） and for 5-Omethylvisammioside from 22. 0 to 110. 0 μg /mL,r = 0. 9996565（n = 6）,the average recovery rate is 100. 28 and 100. 56% respectively. Conclusion： The quantitative analysis of determination of cimicifugoside and 5-O-methylvisammios-De is conducted. The established method with high accuracy is specific,which can be used to determine the contents of cimicifugoside and 5-O-methylvisammios-De in anti-nettle capsules.