摘要
对分离自广西北海红树林根际土壤中的1株放线菌BCG36进行抗菌活性及生理生化特性的研究,并对其进行16S rDNA序列分析。结果显示:放线菌BCG36革兰氏染色呈阳性;有淀粉酶、纤维素酶、脂肪酶活性;牛奶先凝固后冻化,可使硝酸盐还原,不产硫化氢,不产黑色素,不使明胶液化;对大肠埃希菌、普通变形杆菌、乙型溶血性链球菌、金黄色葡萄球菌和白假丝酵母菌均有较好的抑制作用。16SrDNA测序后比对显示,放线菌BCG36菌株属于链霉菌属(Streptomyces)。
Actinomycete BCG36 was isolated from rhizosphere soil of Guangxi Beihai mangrove forest. Antibacterial activity, physiological and biochemical profiles, and 16 S rDNA sequence were tested. The isolate showed gram positive,amylase, cellulase lipase activity and milk coagulation before they were frozen. The tests for nitrate reduction, hydrogen sulfide, melanin, gelatin liquefaction were negative. The 16 S rDNA sequence determination disclosed highly homologous with Streptomyces albidoflavus HD-109. Actinomycete BCG36 exhibited considerable inhibitory effects against E. coli, P.vulgaris, S. hemolyticus, S. aureus, P. aeruginosa, S. pneumoniae and C. albicans.
出处
《广东农业科学》
CAS
CSCD
北大核心
2014年第18期66-69,共4页
Guangdong Agricultural Sciences
基金
广西红树林保护重点实验室(GKLMC-201204)
国家自然科学基金(31260004)
广西自然科学基金(2012GXNSFAA053038)
关键词
红树林
根际放线菌
鉴定
抗菌活性
mangrove forest
rhizosphere actinomycetes
identification
antimicrobial activity
