增液行舟方药对津亏便秘衰老模型水通道蛋白表达的影响

Effects of "Increasing fluid to move the boat" Prescription on the expression of AQP1 in the lung and AQP3 in skin on the constipated aged mice modle with syndrome of fluid-deficiency

目的观察增液行舟方药增液承气汤、增液汤对津亏便秘衰老模型小鼠皮肤、肺组织水通道蛋白(Aquaporin,AQP)表达的影响。方法将小鼠随机分为空白对照组、模型组、西沙必利组、增液承气汤组、增液汤组。采用限水(1.5ml/d),叠加复方地芬诺酯[10mg/(kg·d)]灌胃,同时10%D-半乳糖皮下注射[1000mg/(kg·d)]6周建立便秘衰老模型。从第4周起分别给予相应药物灌胃干预3周。6周后观察整体状态,检测血清、皮肤和肺组织SOD活性,皮肤AQP3及肺组织AQP1的表达。结果与空白组比较,便秘衰老组血清、皮肤和肺组织SOD活性降低,皮肤AQP3、肺组织AQP1表达降低(P<0.01);与便秘衰老组比较,增液承气汤、增液汤组血清、皮肤和肺组织SOD活性升高,皮肤AQP3、肺组织AQP1表达上调(P<0.01)。结论增液承气汤、增液汤可能通过增强机体抗氧化、清除自由基的能力,及抑制便秘导致的AQP表达下降,从而实现津液的濡养作用。

Objective To investigate the effects of ＂ Increasing fluid to move the boat＂ Prescription（Zengyechengqitang and Zengyetang） on the expression of AQP1 in the lung and AQP3 in skin on constipation model mice. Methods The mice of blank group are given ultrapure water every day,while four groups are given 1. 5 ml intragastric administration in sequence as follows：the ultra-pure water（20 ml/kg. d）,diphenoxylate（10 ml/kg. d）,ultra-pure water（to add the remaining amount）,an interval of 3hours every day,simultaneously with 0. 1ml/10 g 1000mg/kg D-galactose in the napes and backs continuously for 42 days to establish the subacute aged constipation models. Since Zengyechengqitang（0. 6g/ml）,Zengyetang（0. 51 g/ml） and Cisapride group（2. 5mg/kg） have been established model for three weeks,we give corresponding gavage therapy with 0. 2ml/10 g for three weeks.After 6 weeks,the changes of the activity of SOD in serum,skin and lung are tested with biochemical assay; the expressions of AQP3 in skin and AQP1 in the lung are detected with Western blot analysis. Results Compared with the blank group,the SOD activity in serum,skin and lung tissue decrease remarkably（P〈0. 01）; the expression level of AQP3 in skin and AQP1 in the lung reduce remarkably（P〈0. 01）. Compared with the aged constipation group,Zengyechengqitang and Zengyetang show the expression level of AQP3 in skin and AQP1 in the lung sharply upregulate（P〈0. 01）; the SOD activity in serum,skin and lung enhance remarkably（P〈0. 01）. Conclusion Zengyechengqitang and Zengyetang can attenuate constipation-induced down-regulation of AQP3 in skin and AQP1 in the lung tissue; increase anti-oxadation activity and free radicals＇ scavenging,and accordingly to achieve transportation of Jin-fluid.