围着床期小鼠子宫内膜中p38MAPK的表达及变化规律

Expression and change regularity of p38MAPK in endometrium of mice during peri- implantation period

目的：检测围着床期小鼠子宫内膜中p38MAPK的表达及变化规律。方法：利用免疫组织化学染色和图像分析法检测动情期未孕、真孕及假孕3-7天小鼠子宫内膜中p38MAPK的表达及变化规律。结果：在真孕组E3天,p38MAPK在小鼠子宫内膜腔上皮细胞中的表达呈阳性,E4天的表达呈强阳性,此后逐渐变弱;p38MAPK在腺上皮中的表达始终保持在阳性水平,而p38MAPK在蜕膜细胞中的表达从E5天起逐渐增强。而p38MAPK在假孕组的表达只有E4天呈弱阳性。结论：1p38MAPK参与胚泡着床过程,并可能参与子宫内膜蜕膜化。2不仅母体因素对子宫内膜中p38MAPK的表达有影响,而且胚泡刺激也对其有影响。

Objective： To detect the expression and change regularity of p38MAPK in endometrium of mice during peri - implantation period. Methods： Immunohistochemical staining and image analysis were used to detect the expression and change regularity of p38MAPK in nonpregnant mice, pregnant mice and pseudopreguant mice during 3 -7 days. Results： In pregnant mouse group, the expression of p38MAPK in luminal epithelium of endometrium was positive on the third day, which was strong positive on the fourth day, then the expression weakened; the expression of p38MAPK in glandular epithelium of endometrium maintained positive; the expression of p38MAPK in decidual cells enhanced gradually from the fifth day. In pseudopregnant mouse group, the expression of p38MAPK was weak positive only on the fourth day. Conclusion： p38MAPK is involved in the implantation process, and it may be involved in the process of en- dometrial decidualization. The expression of p38MAPK in endometrium of mice is affected not only by maternal factors, but also by stimulus of blastocyst.