超敏C反应蛋白及rs1205基因多态性与脑梗死的相关性研究

The relevance study of hyper-sensitive C-reactive protein genes & rs1205 gene polymorphism and cerebral infarction

目的分析并评价超敏C反应蛋白(hs-CRP)及rs1205基因多态性与脑梗死的相关性。方法将113例中老年脑梗死患者作为观察组,113例健康体检者作为对照组。通过免疫透射比浊法测定hs-CRP含量;同时以聚合酶链式反应(PCR)及限制性片段长度多态(RFLP)方法测定rs1205基因的多态性;采用美国国立卫生院神经功能缺损评分(NIHSS)量表评定神经功能。分析各指标的关系。结果 2组的基因型频率与等位基因的分布趋势差异无统计学意义(P均>0.05);重度患者的hs-CRP与log CRP含量均显著高于轻、中度患者(P均<0.05);rs1205基因型与脑梗死病情程度未见明显关联(P>0.05)。结论 hsCRP含量与脑梗死患者病情密切相关,而rs1205基因多态性与脑梗死发病之间未见显著相关性。

Objective： It is to analyze and evaluate the relevance of hyper-sensitive C -reactive protein（hs -CRP） ＆amp;rs1205 gene polymorphism and cerebral infarction.Methods 113 patients with elderly cerebral infarction were selected as ob -servation group,and 113 healthy controls were used as control group .The content of hs -CRP levels was determined by im-mune transmission turbidimetry; At the same time,rs1205 gene polymorphism was examined by polymerase chain reaction （PCR）and restriction fragment length polymorphism （RFLP）methods;Nerve function was evaluated by neurological deficit score of the National Institutes of Health of USA.The relationships among every indexes were analyzed.Results Genotype fre-quency and the distribution trend of alleles of the patients with cerebral infarction of observation group and the subjects of con-trol group were the same, the differences were not significant（P 〉0.05）;hs -CRP and log CRP levels of severe patients were significantly higher than those of mild and moderate patients（P 〈0.05）;There was no obvious correlation between rs1205 gene type and cerebral infarction disease degree.Conclusion The content of hs -CRP is closely related with states of patients with brain infarction, while rs1205 gene polymorphism had no significant relationship with the occurrence of cerebral infarction.