摘要
将氧化石墨烯(GO)在玻碳电极(GCE)表面进行直接电化学还原,再组装上纳米金-壳聚糖(AuNP-CS)聚阳离子,形成了电化学还原氧化石墨烯/纳米金-壳聚糖(ERGO/AuNP-CS)复合膜修饰的玻碳电极。采用扫描电子显微镜(SEM)表征了不同修饰膜表面的形貌,探讨了其对尿酸(UA)分子的差分脉冲伏安(DPV)行为,发现ERGO/AuNP-CS复合膜对UA分子表现出显著的电催化氧化活性。在0.10mol/L磷酸盐缓冲溶液(pH=6.5)中,扫速为100mV/s时,此复合膜修饰电极的DPV响应与UA的浓度在0.05-110μmol/L范围内呈性关系,检测限为12.4nmol/L(S/N=3)。此修饰电极具有良好的选择性、重现性和稳定性,可应用于人体血清和尿液样品中UA的测定,回收率达到93.8%-104.1%。结果与分光光度法和尿酸酶试剂盒法相符。
An electrochemically reduced graphene oxide/gold nanoparticle-chitosan(ERGO/AuNP-CS) composite film modified glassy carbon electrode ( GCE) was constructed by directly electrochemical reduction of GO, and then assembly of AuNP-CS polycation on the surface. The surface morphologies of different modified electrodes including bare GCE, GCE/GO, GCE/ERGO and GCE/ERGO/AuNP-CS were characterized by scanning electron microscopy ( SEM ) . The differential pulse voltammetric behaviors of the electrodes were investigated, and the results indicated that the composite of ERGO/AuNP-CS exhibited excellent electrocatalytic oxidation activity to uric acid ( UA) molecule. In 0. 10 mol/L of phosphate buffer solution (pH=6. 5) with a scanning rate of 100 mV/s, the proposed composite film modified electrode showed a linear electrochemical response to UA in the range of 0 . 05-110 μmol/L with a detection limit of 12. 4 nmol/L ( S/N = 3 ). The electrode displayed good selectivity, reproducibility and stability in the determination of UA in human serum and urine samples with a recovery of 93 . 8%-104 . 1%. The detection results were agreed with those of conventional spectrophotometry and uricase Kit methods.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2014年第11期1656-1660,共5页
Chinese Journal of Analytical Chemistry
基金
国家科技支撑计划(Nos.2012BAD31B08,2012BAC17B01)
国家自然科学基金(Nos.21275022,21105005)
湖南省科技计划(No.2013FJ3075)项目资助~~
关键词
电化学还原氧化石墨烯
纳米金-壳聚糖聚阳离子
尿酸
电催化氧化
修饰电极
Electrochemically reduced graphene oxide
Gold nanoparticle.chitosan polycation
Uric acid
Electrocatalytic oxidation
Modified electrode