一种保留胶原天然结构的新型生物表面活性剂的性能表征

Characterization of a Novel Biosurfactant Based on Collagen Retaining its Native Structure

研究了一种保留胶原天然结构的生物表面活性剂的表面张力等基本物化性能。由红外光谱和凝胶电泳分析表明改性后的胶原仍保留天然胶原的三股螺旋结构,但分子量相对于天然胶原稍有增加。改性胶原的临界胶束浓度(CMC)为5.0×10-3mg/m L,在CMC时表面张力为50.5 m N/m,具有良好的表面活性。改性胶原的等电点由天然胶原的7.06下降到5.26,具有水溶性。其变性温度比天然胶原提高了7℃。原子力显微镜观察改性胶原仍具有天然胶原的纤维结构,但纤维直径略有增大。

The basic physicochemical properties（surface tension etc） of a biosurfactant based on collagen retaining its native structure were studied in this paper. The results of infrared spectroscopy and electrophoresis analyses demonstrated that the modified collagen still retained its triple helix structure, but the molecular weight of the modified collagen increased slightly than that of native collagen. The critical micelle concentration（CMC） of the modified collagen was 5.0 ×10^-3mg/m L and the surface tension at CMC was 50.5 m N/m. Thus the modified collagen had a good surface activity. The isoelectric point of the modified collagen decreased to 5.26 from that（7.06） of native collagen, indicating the water solubility of the modified collagen. The denaturation temperature of the modified collagen was about 7.0 ℃ higher than that of native collagen. As observed by atomic force microscopy, the modified collagen still possessed the fibrous structure of native collagen, but the fiber diameter increased slightly after modification.