产铁载体菌的筛选鉴定及活化镉的性能探究

Isolation, Identification and Study on Cadmium-Mobilization Ability of Sidorophores-producing Bacteria

从湘江流域某冶炼厂周边土壤中筛选出高抗镉微生物,在镉离子浓度为2 mmol/L的培养基中正常生长。通过改进的CAS平板上检测出2株具有铁载体产生能力的细菌H1和H2。H1产物具有较强的荧光性能,而且H1菌对重金属Cd有较强的活化能力。接种4 d后,土壤中可溶性镉的含量(21 mg/kg)是对照组(10.4 mg/kg)的2倍多。用扫描电子显微镜观察显示其形态与假单胞菌类似;其16S rDNA序列与Pseudomonas sp.序列相似度达99%。

The cadmium-resistant（Cd） strains which can normal grow in the concentration of 2 mmol/L Cd medium were isolated from metal contaminated soil around a smeltery beside Xiangjiang River in China. Then sidorophores-producing bacteria（SPB） H1 and H2were screened out using the modified Chrome Azurol S（CAS）-agar plate. The product of H1 with strong fluorescence intensity can mobilize Cd in soils. After 4 d vaccination, the concentration of soluble cadmium with H1（21mg/kg） was more than twice as higher than the control group（10.4 mg/kg）. H1 strains have the same morphology with Pseudomonas, and 16 S rDNA sequence has 99% similarity to Pseudomonas sp.