湖北地区宫颈疾病患者HPV基因型及其年龄分布

Investigation and Analysis of HPV Infection Status in Patients with Gynecological Disease in Hubei Province

目的:分析湖北地区宫颈疾病患者人乳头状瘤病毒(HPV)感染的基因型分布及与年龄的关系。方法:4 314例宫颈疾病患者,年龄21-86岁,按5岁一组分为10组,收集各组宫颈脱落细胞样本,应用PCR-基因芯片技术进行HPV分型检测,统计分析各组HPV总感染率和各亚型感染率及其年龄分布特点。结果:HPV总感染率为28.86%(1 245/4 314)。以单纯高危型HPV(hrHPV)为主,感染率为23.78%(1 026/4 314),单纯低危型HPV(lrHPV)感染率只占2.30%(99/4 314),余为混合感染。高危型中又以HPV16型最多,感染率为9.23%(398/4 314),其次为HPV52和58型,感染率为5.42%(234/4 314)和4.75%(205/4 314)。HPV总感染率以21-25岁组(29.97%)和51-55岁组(31.75%)最高,最低为31-35岁组(25.19%)和>65岁组(25.42%),但各组间差异无显著性(P>0.05)。单一感染率明显较多重感染率高(20.91%vs 7.95%,P<0.01)。结论:加强湖北地区宫颈疾病患者HPV感染的筛查和监测,有利于该地区女性人群生殖器疣和宫颈癌防治以及流行病学研究。

Objective：To investigate the distribution of different human papillomavirus （HPV）subtypes in-fection in patients with gynecological disease in Hubei province.Method：A total of 4 314 cases were recruited and the cervical cell samples were collected for detection for HPV genotypes by using PCR and gene chip.Results：1 245 out of 4 314 cases were detected with HPV-positive infection （28.86%,1 245/4 314）,1026 cases were detected with only high risk HPV （hrHPV）infection （23.78%,1026/4 314）,99 cases were detected with only low risk HPV （lrHPV）infection （2.30%,99/4 314）and the rest were detected with both hrHPV and lrHPV infection. The most prevalence hrHPV subtype were HPV16 （9.23%,398/4 314）,HPV52 （5.42%,234/4 314）and HPV58 （4.75%,205/4 314）.The 21-25 group （29.97%）and 51-55 group （31.75%）had high prevalence while 31-35 group （25.19%）and 〉65 group had low prevalence.But there was no significant difference （P〉0.05）between two groups.Single HPV type infection rate was significantly higher than multiple HPV type infection rate （20.91%vs 7.95%,P〈0.01）.Conclusion：It is necessary to screen HPV for prevention of genital warts and cervical cancer in Hubei region.