穴位局部P物质与肥大细胞在针刺效应启动中作用的研究

Study of the Roles of Acupoint Substance P and Mastocytes in Starting the Acupuncture Effect

目的观察足三里穴位局部P物质对急性胃损伤小鼠胃排空率的影响,以及不同干预措施下胃损伤小鼠穴位局部SP表达与肥大细胞(MC)功能的相关性。方法将200只昆明系雄性小鼠随机分为正常组、正常针刺组、模型组、模型针刺组、模型PBS穴位注射组、模型SP穴位注射组、模型spantide(P物质拮抗剂,[D-Arg1,D-Trp7,9,Leu11]-Substance P)穴位注射组、模型PBS穴位注射后针刺组、模型SP穴位注射后针刺组、模型spantide穴位注射后针刺组,每组20只。以盐酸灌服法复制小鼠急性胃损伤模型,在肯定针刺足三里穴促进模型小鼠胃排空率的基础上,观察不同干预措施下胃损伤小鼠穴位局部SP表达与MC功能相关性。结果针刺足三里穴可明显增加胃损伤小鼠胃排空率,穴位注射SP拮抗剂后针刺促胃损伤小鼠胃排空率作用消失;胃损伤状态下穴位局部SP表达与MC脱颗粒率均升高;穴位注射P物质或针刺均可增强SP阳性指数表达;穴位注射0.9?g P物质后再针刺可使SP阳性指数表达下调;穴位注射1.0?g spantide拮抗穴区SP再针刺,SP阳性指数表达明显降低,甚至低于正常组;穴位注射0.9?g SP促MC脱颗粒效应强于针刺作用,当两者共同作用时,却可使脱颗粒MC数与MC脱颗粒率呈下降趋势。结论穴位处SP与MC之间的相互作用可能是针刺效应启动中的关键因素。

Objective To investigate the effect of substance P at point Zusanli（ST 36） on gastric emptying rate and the correlation between acupoint substance P expression and mast cell function under different interventions in mice with acute gastric injury. Methods Two hundred Kunming male mice were randomly allocated to normal, acupuncture-normal, model, acupuncture-model, PBS acupoint injection-model, SP acupoint injection-model, spantide（substance P antagonist, [D-Arg1, D-Trp7,9, Leu11]-Substance P） acupoint injection-model, after-PBS acupoint injection acupuncture-model, after-SP acupoint injection acupuncture-model and after-spantide acupoint injection acupuncture-model groups, 20 mice each. A mouse model of acute gastric injury was made by an oral gavage of hydrochloric acid. The correlation between acupoint substance P expression and mast cell（MC） function was investigated under different interventions in mice with acute gastric injury after the promoting effect of point Zusanli（ST 36） acupuncture on gastric emptying was determined in the mouse model. Results Acupuncture at point Zusanli（ST 36） markedly increased gastric emptying rate in gastric injury mice. The increasing effect of acupuncture on gastric emptying rate disappeared in gastric injury mice after acupoint injection of substance P antagonist. Both substance P expression and MC degranulation rate at the acupoint increased in a state of gastric injury. Acupoint injection of substance P or acupuncture increased the expression of SP positive index. Re-acupuncture after acupoint injection of 0.9 ？g substance P down-regulated the expression of SP positive index. The expression of SP positive index decreased markedly or was even lower than in the normal group following re-acupuncture after 1.0 ？g spantide counteracted acupoint SP. The promoting effect of acupoint injection of 0.9 ？g SP on MC degranulation was stronger than that of acupuncture. When the two acted together, MC degranulation number and rate had a decreasing tendency. Conclusion The interaction between acupoint SP and MC may be a key factor in starting the acupuncture effect.