林可霉素对小鼠肠道屏障作用的影响

Effect of Lincomycin on the Intestinal Mucosa Barrier of Mice

目的观察盐酸林可霉素对小鼠肠黏膜屏障的损伤作用。方法将24只小鼠均分为正常对照组和造模组。造模组给予盐酸林可霉素灌胃,建立小鼠肠黏膜破损及肠道菌群失调模型。检测两组小鼠肠道菌群,分析细菌易位及通透性情况[检测血浆二胺氧化酶(DAO)]。结果小鼠灌服盐酸林可霉素3 d,肠道菌群失调。正常对照组需氧菌易位率16.7%,造模组52.3%;正常对照组厌氧菌易位率8.3%,造模组68.2%。上述指标比较,造模组均显著高于正常对照组,且差异有统计学意义(P<0.01)。正常对照组血浆DAO(2.08±0.05)mg·m L-1,造模组(7.18±0.10)mg·m L-1,造模组高于正常对照组,差异有统计学意义(P<0.01)。结论盐酸林可霉素对小鼠肠黏膜屏障有损伤作用。

Objective To study the effect of lincomycin hydrochloride on the intestinal mucosal barrier of mice.Methods A total of 24 mice were equally and randomly divided into normal control group and model group. Mice in the model group were administered with 8 mg · kg^- 1·d^- 1of lincomycin hydrochloride through gastric lavage for 3 days to establish the model of intestinal mucosa injury and alteration of intestinal flora in mice. The intestinal flora was tested,bacterial translocation was analyzed,and mucosa permeability was determined by measuring diamine oxidase（ DAO） in plasma. Results Lincomycin hydrochloride led to alteration of intestinal flora in mice after oral administration for three days in mice. The aerobes translocation rate was 16. 7% and 52. 3% in the normal and model groups,respectively（ P〈 0. 01）. The anaerobes translocation rate was8. 3% and 68. 2% in the normal and model groups,respectively（ P〈 0. 01）. The plasma concentration of diamine oxidase was increased from（ 2. 08 ± 0. 05） mg·m L^- 1in the normal group to（ 7. 18 ± 0. 10） mg·m L^- 1in the model group（ P〈 0. 01）.Conclusion Lincomycin hydrochloride damages intestinal mucosal barrier in mice.