Nanog表达上调促进乳腺癌细胞MCF-7的增殖和侵袭

Up-regulation of Nanog promotes cell proliferation and invasion in breast cancer cells MCF-7

目的:观察Nanog高表达对乳腺癌细胞MCF-7生物学特性的影响。方法:构建重组质粒pcDNA3.1(-)-Nanog,利用免疫荧光观察Nanog高表达后,乳腺癌细胞MCF-7中Nanog的定位。利用平皿克隆分离法获得Nanog高表达的乳腺癌细胞系MCF-7。应用克隆形成实验、Transwell迁移和侵袭实验观察Nanog高表达对乳腺癌细胞MCF-7生物学特性的影响。结果:Nanog在MCF-7细胞中定位于核,Nanog高表达后,乳腺癌细胞MCF-7的增殖、迁移和侵袭能力显著增强。结论:Nanog能够促进乳腺癌细胞MCF-7的增殖、迁移和侵袭能力。

Objective： To observe the effects of up-regulation of Nanog on the biological characteristics of breast cancer cells MCF-7.Methods： A recombinant plasmid pc DNA3.1（-）-Nanog was constructed. Using immunofluorescence, Nanog positioning in MCF-7 was observed after transfected with plasmid pc DNA3.1（-）-Nanog. Breast cancer cell line MCF-7 high expression Nanog was obtained by using cloning separation plate. The effects of up-regulation of Nanog on the biological characteristics of MCF-7 breast cancer cells were observed by clonogenic assay, Transwell migration and invasion assay. Results： Nanog was localized in the nucleus in MCF-7 cells. MCF-7 high expression of Nanog had more significant effect on cell proliferation, migration and invasion. Conclusion： Up-regulation of Nanog could promote cell proliferation, migration and invasion of MCF-7.