反相高效液相色谱法测定保婴镇惊丸中大黄素和大黄酚含量

Content Determination of Emodin and Chrysophanol in Baoyingzhenjing Pill by RP- HPLC

目的：建立测定保婴镇惊丸中大黄素和大黄酚含量的反相高效液相色谱法。方法色谱柱为Zorbax SB－C18柱（250 mm ×4.6 mm，5μm），流动相为甲醇－0.1%磷酸（85：15），检测波长为254 nm，柱温为35℃。结果大黄素、大黄酚进样量分别在0.0412~0.6180μg （ r=0.999999）和0.0906~1.3590μg（ r=0.999998）范围内与峰面积线性关系良好；平均回收率分别为97.02%和98.12%，RSD分别为1.16%和0.94%（ n=6）。结论该方法简便、快速、准确，适用于保婴镇惊丸中大黄素、大黄酚的含量测定。

Objective To establish a RP-HPLC method for the content determination of emodin and chrysophanol in Baoyingzhenjing Pill. Methods The analysis was performed on the Zorbax SB-C18 column （ 250 mm × 4. 6 mm,5 μm ） with methanol-0. 1% phosphoric acid（85 ： 15） as the mobile phase. Emodin and chrysophanol were detected at 254 nm. The column temperature was 35 ℃. Results The linear range was 0. 041 2-0. 618 0 μg（ r=0. 999 999）for emodin sample size and 0. 090 6-1. 359 0 μg（ r=0. 999 998）for chrysophanol sample size,respectively. The average recovery rates of emodin and chrysophanol were 97. 02% and 98. 12%,RSD were 1. 16% and 0. 94% （ n=6 ） ,respectively. Conclusion This method is simple,rapid,accurate and suitable for determining the contents of emodin and chrysophanol in Baoyingzhenjin Pill.