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光谱联合电泳法研究双酚A与肿瘤相关DNA的相互作用 被引量：1

Studies on the Interaction of BPA with Tumor Related DNA by Spectroscopic and Electrophoresis Methods
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摘要 研究双酚A与人类肿瘤相关DNA(抑癌基因p53 DNA和癌基因C-myc DNA)的相互作用。分别采用紫外、荧光和圆二色等光谱方法和聚丙烯酰胺凝胶电泳法研究了双酚A与人类肿瘤相关DNA(抑癌基因p53 DNA和癌基因C-myc DNA)的相互作用模式。其中紫外、荧光和圆二色滴定实验均表明了嵌插作用的存在,离子强度实验、KI猝灭对比实验和凝胶电泳实验证实了静电和嵌插作用的共同作用,可进一步影响DNA的双螺旋构象。 A variety of spectroscopic methods and polyacrylamide gel electrophoresis method were used to study the interaction of bisphenol A and human tumor related DNA （p53 DNA and C-myc DNA）. In which, UV, fluorescence and circular dichroism titration experiments showed the presence of intercalation interaction, ionic strength experiment, KI quenching experiment and gel electrophoresis experiment confirmed that bisphenol A bound to DNA via electrostatic binding and intercalation, which can affect the normal conformations of DNA.
作者 李莉 鲁嘉 李卉卉 杨小弟
机构地区 江苏大学分析测试中心 安徽中医药大学药学院 江苏省生物医药功能材料协同创新中心
出处 《化学分析计量》 CAS 2014年第6期37-41,共5页 Chemical Analysis And Meterage
基金 水利部公益性行业科研专项经费项目(201201018) 江苏高校优势学科建设工程项目资助
关键词 人类肿瘤 双酚A DNA 相互作用 human tumor bisphenol A DNA interaction
分类号 O657 [理学—分析化学]
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参考文献14

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二级参考文献45

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化学分析计量
2014年 第6期

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