2012年-2013年杭州地区婴幼儿病毒性腹泻监测结果分析

Analysis of viral diarrhea surveillance results amomg infants in Hangzhou from 2012 to 2013

目的了解杭州地区婴幼儿病毒性腹泻的流行现状。方法收集浙江省儿童医院2012年-2013年5岁以下腹泻门诊患儿粪便标本,采用实时荧光定量PCR方法同时进行轮状病毒(RV)、诺如病毒(NV)、星状病毒(ASV)、札如病毒(SAV)和肠道腺病毒(EADV)核酸检测。结果 307份粪便标本中,5种病毒阳性率分别为21.17%、17.92%、0.33%、3.58%和3.91%,123份标本含有至少1种病毒核酸,其中103份为单一病毒核酸阳性,20例为混合感染。结论2012年-2013年杭州市婴幼儿病毒性腹泻呈高发状态,轮状病毒是主要病原体,其次是诺如病毒,札如病毒和肠道腺病毒也占一定的比例,星状病毒感染较少,另外还有混合感染现象。今后应加强婴幼儿病毒性腹泻的监测,并关注混合感染。

Objective To investigate the epidemic status of viral diarrhea amomg infants in Hangzhou.Methods Stool samples were collected from the diarrhea infants aged less than 5 years in children＇ s hospital of Zhejiang province from 2012 to2013.Real-time fluorescent quantitative PCR（RT-FQ-PCR） was used to detect the nucleic acid of rotarirus（RV）,norovirus（NV）,astrovirus（ASV）,spav（SAV） and enteric adenovirus（EADV）.Results In 307 stool samples,the positive rate was 21.17% for rotarirus,17.92% for norovirus,0.33% for astrovirus,3.58% for spav and 3.91% for enteric adenovirus.123 specimens contained at least one virus nucleic acid.Among them,103 for a single positive and 20 for co-infections.Conclusion The incidence of viral diarrhea amomg infants was high in Hangzhou from 2012 to 2013.Rotarirus was the most prevalent pathogen,followed by norovirus,spav and enteric adenovirus was detected too,astrovirus infection was rare.In addition,there was phenomenon of co-infections.It is necessary to strengthen the surveillance of viral diarrhea amomg infants and pay close attention to co-infections in the futher.