2011年-2013年泰安市婴幼儿食品食源性致病菌污染状况调查

Investigation on contamination of food borne pathogens in infants foods of Tai'an from 2011 to 2013

目的了解泰安市婴幼儿食品食源性致病菌污染状况,为婴幼儿食源性疾病预警及食品安全管理提供科学依据。方法依据GB4789-2010及GB/T4789-2003检测程序对婴幼儿食品(包括奶粉、米粉、营养饼干、面制品4类)进行腊样芽孢杆菌、阪崎肠杆菌、金黄色葡萄球菌的分离培养及鉴定。结果 2011年-2013年220份样品中共检出致病菌34株,总检出率为15.5%(34/220)。其中腊样芽孢杆菌检出27株,检出率为12.3%(27/220);阪崎肠杆菌检出5株,检出率为2.3%(5/220);金黄色葡萄球菌检出2株,检出率为0.9%(2/220),不同致病菌检出率差异有统计学意义(χ2=34.668,P<0.001)。奶粉、米粉、营养饼干、面制品4类婴幼儿食品的致病菌检出率分别为:22.9%(16/70)、14.3%(8/56)、10.5%(6/57)、10.8%(4/37)。结论泰安市婴幼儿食品存在食源性致病菌的污染,其中奶粉的污染较为严重,腊样芽孢杆菌是主要病原菌。

Objective To investigate the contamination of food borne pathogens from infants foods in Tai＇ an,so as to provide some scientific basis for early warning of infants food borne disease and control of food safety management.Methods According to GB4789-2010 and GB/T4789-2003 standard method,we performed iaolatioin,biochemical identification and serological identification of Bacillus cereus,Enterobacter sakazakii,Staphylococcus aureus,in four kinds of infants foods samples,including milk powder,ground rice,nutrient biscuit and flour products.Results We had checked up 220 samples from 2011 to2013,as a result,there were 34 pathogenic bacteria to be found,（total detection rate was 15.5%,34/220）,involved 27 Bacillus cereus（with detection rate 12.3%,27/220）,5 Enterobacter sakazakii（with detection rate 2.3%,5/220）,2 Staphylococcus aureus（with detection rate 0.9%,2/220）.There was significant difference of the contamination rates in 3 pathogenic bacteria（χ^2= 34.668,P 0.001）.The contamination rate detected in milk powder was 22.9%（16/70）,ground rice 14.3%（8/56）,nutrient biscuit 10.5%（6/57）,flour products 10.8%（4/37）.Conclusion Food borne pathogens contamination existed in infants food in Tai＇an,and milk powder is the main contamination food,while the main pathogenic is bacteria Bacillus cereus.