子宫内膜异位症中miR-556-3p的异常表达及其与血管内皮生长因子的关系

Dysregualtion of micro RNA-556-3p may contribute to the pathogenesis of endometriosis by targeting the expression of VEGF

目的探索micro RNA-556-3p(mi R-556-3p)在子宫内膜异位症中的表达模式及其与血管内皮生长因子(vascular endothelium growth factor,VEGF)的关系。方法子宫内膜异位症患者15例作为研究组,分别收集在位内膜、卵巢异位内膜及腹膜异位内膜;无子宫内膜病变的妇女16例,收集子宫内膜组织作为对照组。Taqman real-time PCR检测上述组织中mi R-556-3p的水平,Western Blot法检测组织中VEGF蛋白含量,并分析mi R-556-3p与VEGF表达水平之间的相关性。结果与对照组相比较,内异症患者在位内膜及腹膜异位病灶中mi R-556-3p水平显著降低(P<0.001),而VEGF含量在内异症在位内膜、卵巢异位囊肿或腹膜异位内膜中均显著升高(P值分别为<0.001、0.02、<0.001);与内异症组在位内膜相比较,卵巢异位病灶中mi R-556-3p显著升高(P<0.001),腹膜异位病灶中mi R-556-3p的表达量则显著降低(P<0.001),而VEGF显著升高(P<0.001);异位症组在位内膜及腹膜异位病灶中mi R-556-3p与VEGF蛋白的表达量呈显著负相关(r值分别为-1.026、-1.819,P值分别为0.01、0.04)。结论内膜组织中mi R-556-3p表达异常可能导致VEGF蛋白表达失调,参与子宫内膜异位症的发生发展。

Objective：To determine the dysregulation of micro RNA-556-3p expressions in eutopic and ectopic endometria from endometriosis patients as well as their relationships with the levels of VEGF protein. Method：Fifteen women with endometriosis were recruited as the study group,whose eutopic endometria,ovarian and peritoneal endometriotic lesions were excised. Endometrial tissues from sixteen women without endometriosis were collected as the control group. Taqman real-time PCR was performed to detect the expression levels of mi R-556-3p in the above groups,and Western blot was used to examine the protein levels of VEGF. Moreover,the correlation of VEGF protein with mi R-556-3p levels was analyzed. Results：Compared with the control group,the expressions of micro RNA-556-3p in eutopic endometria and peritoneal lesions were significantly elevated（P〈0.001）,with statistically significant decreases in VEGF protein levels in all the eutopic or ectopic endometrial tissues（P〈0.001,P=0.02,P〈0.001 respectively）. Besides,the ovarian endometriotic lesions had a higher level of mi R-556-3p than eutopic endometrial（P〈0.001）. Whereas,the downregulation of mi R-556-3p and overexpression of VEGF protein were observed in peritoneal lesions compared with the eutopic endometrial tissues（P〈0.001）. Moreover,the levels of mi R-556-3p were inversely correlated with the VEGF protein levels in both eutopic endometria and peritoneal endometriotic lesions（r=-1.026,P=0.01;r=-1.819,P=0.04,respectively）. Conculsion：Dysregulation of mi R-556-3p might cause the abnormal expression of VEGF and contribute to the pathogenesis of endometriosis.