马钱子配伍白芍对小鼠LD_(50)和镇痛作用的影响

Effect of Compatibility of Nux Vomica and Radix Paeoniae Alba on LD_(50) and Analgesic Effect of Mice

目的 :探讨马钱子配伍白芍(以下简称马白)前后毒性及镇痛作用的变化,寻求最佳配伍比例。方法 :将小鼠随机分为空白对照组,马钱子单煎剂组,马白1∶2、1∶4、1∶6煎剂组,利用急性毒性实验,分别测得马钱子单煎剂和马钱子白芍各配伍组的LD50;利用热板法和冰醋酸所致小鼠扭体法观察马白配伍前后镇痛作用的变化。结果 :马白组与马钱子单煎剂组比较,毒性降低,且以马白1∶6减毒效果较佳。镇痛作用中马白组优于单煎剂组,马白1∶6镇痛作用较好。结论:白芍降低马钱子毒性作用明显,可增强其镇痛作用,有减毒增效作用,且以马白1∶6为宜。

Objective ：To discuss the compatibility of Nux vomica and Radix Paeoniae Alba（abbreviated as N-R） on the toxicity and analgesic effect changes in order to finds out optimum compatibility ratio. Meth- ods： The mice were randomly divided into blank control group,Nux vomica single decoction group,N-R 1： 2,1：4,1：6 decoction group. The LDso of all compatibility groups were measured using acute toxicity experi- ments. Using the hot-plate and acetic acid-induced writhing methods,to observe the analgesic effect changes which before and after the compatibility. Results：Comparing the compatibility groups with Nux vomica single decoction group,the research finds the toxicity can be reduced and the proportion of 1：6 was better. The analgesic effect of the compatibility group was better than the single decoction group,the superior compatibili- ty ratio was N-R 1：6. Conclusion：The experimentation confirms that the Radix Paeoniae Alba can significantly reduce the toxicity of Nux vomica and enhances its analgesic effect. The optimum compatibility ratio was N-R 1：6.