实验小鼠体内曼氏裂头蚴移行途径及分布的研究

Migration and Distribution of Spirometra mansoni in Experimentally Infected Mice

从王锦蛇Elaphe carinate体内检取曼氏裂头蚴Spirometra mansoni,口服法以5条/只感染昆明小鼠,于感染后10 min、20 min、30 min、1 h、2 h、4 h、6 h、24 h、7 d、14 d分别处死一组小鼠(3只小鼠/组),观察裂头蚴在小鼠体内的移行及分布。发现小鼠经口服感染裂头蚴后,裂头蚴最早于10 min穿透胃肠壁;感染后20 min,腹腔内已见穿过肠壁的裂头蚴;感染后10 min至6 h,裂头蚴主要见于胃肠腔、胃肠壁和腹腔内;感染24 h,有少数的裂头蚴移行至小鼠皮下;感染7 d后,大多数的裂头蚴移行至皮下组织,从颈部、躯干及头部皮下组织检获的裂头蚴数分别为7条、6条和1条;脑、心脏、肝脏、肾脏及肺等器官未见裂头蚴寄生。小鼠感染曼氏裂头蚴后,裂头蚴经胃肠壁进入腹腔,随后移行至身体的组织器官内寄生,其中以皮下组织多见。

Pleroceroids Spirometra mansoni were obtained from the wild snakes Elaphe carinate. A total of 30 Kunming mice（ 20 - 25 g） equally divided into 10 groups,and fed each with 5 pleroceroids（ 3 mice / each group） and killed at designed time points from 10 minutes to 14 days. Autopsy was performed on each mouse to recover the pleroceroids. The results indicated that the pleroceroids penetrated into the stomach or small intestine wall as early as 10 minutes after infection,and the process could be finished within 20 minutes and subsequently appeared in abdominal cavity. After 10 minutes to 6hours,most pleroceroids appeared in gastrointestinal lumina,gastrointestinal walls and abdominal cavities of mice. After 1day post infection,a few pleroceroids migrated to subcutaneous tissue and most pleroceroids appeared in subcutaneous tissues within a week. A total of 14 pleroceroids were found in subcutaneous tissues,and among of which,7 were observed around neck region,6 around trunk and 1 in the head of mice. No pleroceroid was found in the brain,heart,liver,kidney and lung tissues of infected mice.