外源性硫化氢对血管性痴呆模型大鼠学习记忆的影响

Effects of exogenous hydrogen sulfide on vascular dementia rat model of learning and memory

目的观察外源性硫化氢(Hydrogen Sulfide,H2S)对血管性痴呆大鼠(Vascular Dementia,Va D)学习记忆改善情况及血管性痴呆大鼠血浆硫化氢含量的变化。方法采用改良的四血管法(4-VO)复制大鼠Va D模型,将模型大鼠按给药干预时间分为1 d组、7 d组、30 d组,每组分为假手术组、模型组、低剂量组、高剂量组。低剂量组每日腹腔注射H2S供体Na HS 30μmol/kg,高剂量组每日腹腔注射H2S供体Na HS 100μmol/kg,假手术组与模型组每日腹腔注射等量生理盐水,然后进行Morris水迷宫检测大鼠学习记忆能力,用HE染色观察海马神经元的损伤情况,并测定Va D大鼠模型血浆硫化氢含量。结果与假手术组相比,模型组逃避潜伏期明显增长(P<0.05),血浆硫化氢浓度明显减少(P<0.05),海马神经元损伤严重;与模型组相比,低剂量组与高剂量组逃避潜伏期明显缩短(P<0.05),血浆硫化氢浓度明显增加(P<0.05),海马神经元损伤减轻,血管性痴呆大鼠学习记忆有所改善。结论硫氢化钠可改善Va D大鼠的认知水平和病理变化,其保护作用可能与提高硫化氢含量有关。

Objective To observe the effcets of exogenous hydrogen sulfideand （H2S） on learning and memory function and the Changes of H2S content of plasma in vascular dementia（VaD） rats. Methods A modified four vessels occlusion method （4-VO） was used for VaD model rats preparation,the rats were divided into three groups by drug intervention time：one day group, seven days group, 30 days group. Every group were divided into four subgroups：sham-operated group, model group,low-dose group, high-dose group. The rats of low-dose group and the high-dose group were intraperitoneallyinjeeted with NariS （ H2S donor） 30 μmol/kg and 100 μmol/kg per day, and the sham-operated group and model group were intraperitoneally injected with saline. Then the Morris water maze test was used to detect the learning and memoryabilities of the rats, the damage of hippocampal neurons was observed by HE, meanwhile, the H2S content in rats plasma was detected. Results Compared with sham-operated group, the model group rats had significantlyprolonged escape latenties（ P 〈 0.05 ） , plasma H2S content reduced obviously （ P 〈 0.05 ） , the damage of hippocampal neurons were observed ; Compared withmodel group, low-dose group and high-dose group rats had signifieantlyshortened escape latencies （ P 〈 0.05 ） , plasma H2S content raised obviously （ P 〈 0.05 ） , the damage of hippocampal neurons were remited, the cognitive disorder significantly alleviated. Conclusion Naris may improve cognitive level andpathological changes in VaD rats, the protective effect may be related to the increase of plasma H2S content.